Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608349
Poster Session
Georg Thieme Verlag KG Stuttgart · New York

Protective effect of Aronia melanocarpa (Chokeberry) extracts from benign prostatic hyperplasia in rats.

NH Kim
1   Gyeongnam Department of Environment & Toxicology, Korea Institute of Toxicology, 660 – 844, Jinju, Korea, Republic of (South)
,
JD Heo
1   Gyeongnam Department of Environment & Toxicology, Korea Institute of Toxicology, 660 – 844, Jinju, Korea, Republic of (South)
,
JH Kim
1   Gyeongnam Department of Environment & Toxicology, Korea Institute of Toxicology, 660 – 844, Jinju, Korea, Republic of (South)
,
KH Hwang
1   Gyeongnam Department of Environment & Toxicology, Korea Institute of Toxicology, 660 – 844, Jinju, Korea, Republic of (South)
,
EJ Jeong
2   Gyeongnam National University of Science and Technology, College of Life Science and Natural Resources, 660 – 758, Jinju, Korea, Republic of (South)
› Author Affiliations
Further Information

Publication History

Publication Date:
24 October 2017 (online)

 

The target of the study is Aronia melanocarpa (Chokeberry), which is originate from the east province of North America and rich of phenolic compounds. In this study, Aronia extracts were treated orally in benign prostatic hyperplasia (BPH) rats for identifying its protective effects.

Accelerated solvent extraction is applied for the extraction of Aronia melanocarpa. Four extract samples (M2, M4, M9 and M10) at 30 °C with 60% ethanol were selected, which showed the potent inhibition on 5-alpha reductase activity in vitro. For in vivo experiments, 8-week old male SPF wistar rats were purchased (n = 30). All animals were castrated, then randomly divided into 6 groups (VC; vehicle control, PC; Saw palmetto extract, T1; M2, T2; M4, T3; M9, T4; M10). After a week of recovery phase, 100 mg/kg of the test materials were orally administered once-a-day for 6 weeks as well as subcutaneous injection of 3 mg/kg testosterone. Prostate glands were collected and measured at necropsy for calculating the Prostate Index. Blood serume chemistry, ELISA and qPCR were also perfomed.

As for the Prostate Index, those of M4 group showed the least value, although it was not statically significant compare to the VC. M2 group was revealed that tissue DHT levels were significantly decreased (141.42 ± 27.75pg/50 mg tissue) and prostatic 5-alpha reductase level showed the lowest in M2 group (167.50 ± 20.48), but not statistically significant. In the qPCR results, M2 and M4 group showed sharp decrease of AR, PCNA and PSA expression, but only PCNA levels were significantly different (VC: 55.08 ± 43.87%, M2: 12.05 ± 3.92%, M4:12.02 ± 12.10% normalize with GAPDH). Additionally, administration of all four Aronia extracts could significantly lower the serum AST level. M9, M10 and Saw palmetto extracts treatment could not contribute to anymeaningful effects.

In this study, it was somewhat insufficient result since many parameters were statistically unclear. However, it was performed with only low concentration of extracts, so that it still have potentials of lowering the risk of BPH.