Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608235
Poster Session
Georg Thieme Verlag KG Stuttgart · New York

Development and validation of an HPLC-DAD method for iridoid glycoside screening in fresh plant parts from Plantago lanceolata L.

E Lotter
1   University of Regensburg, Institute of Pharmacy, Department of Pharmaceutical Biology, 93053 Regensburg, Germany
,
J Heilmann
1   University of Regensburg, Institute of Pharmacy, Department of Pharmaceutical Biology, 93053 Regensburg, Germany
,
D Kammerer
2   WALA Heilmittel GmbH, 73087 Bad Boll/Eckwälden, Germany
,
F Stintzing
2   WALA Heilmittel GmbH, 73087 Bad Boll/Eckwälden, Germany
› Author Affiliations
Further Information

Publication History

Publication Date:
24 October 2017 (online)

 

Plantago lanceolata L. is a long-known medicinical plant, exhibiting multiple favorable properties like anti-bacterial and abbiritative effects.[1] Its drug has been thoroughly analyzed. However, scientific data of the fresh plant are still rare. Therefore, an HPLC-DAD fingerprint method for fresh plant parts from P. lanceolata L. was developed and validated. This method enables simultaneous screening of two substance classes characteristic of Plantago: the phenylethanoids and the iridoid glycosides. According to literature, P. lanceolata herb contains five phenylethanoids[2]: the predominant phenylethanoid acteoside, its isomer isoacteoside, plantamajoside, lavandulifolioside and cistanoside F;[2] as well as six iridoid glycosides: the main iridoid glycosides aucubin[3,4] and catalpol[3,4], together with asperuloside[3,5], globularin[3], verbenalin[4] and the methylester of deacetylasperuloside acid[3]. Among the predominant representatives the following constituents were identified in the fresh plant: the phenylethanoids isoacteoside and plantamajoside as well as the iridoids verbenalin and asperuloside. During validation linearity, intra- and interday precision, robustness, recovery, LOD and LOQ were determined for aucubin at 195nm, previously being detected at 204nm or higher wavelengths[6,7]. Due to the aromatic nature of the phenylethanoids, their detection can easily be performed at 330nm. Consequently their validation was abandoned. The obtained results confirm the validity of the novel HPLC-DAD method and underline its potential applicability in the routine quality control of iridoid containing plant raw materials and preparations derived therefrom.

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[7] Kim et al, Chromatographia, 2009, 69, 11, 1397 – 1400.