Effect of fermented ginseng berry extract, enriched in Ginsenoside Rg2 on suppressing melanin and whitening activity in HDF and B16BL6

This study was carried out to determine the time of fruit harvest which does not affect the growth of 4 years and 5 years ginseng roots for the commercialization of fruit as a by – product of ginseng. Saponin content, total phenolic compound, acid polysaccharide content and antioxidant effect were determined in each sample during the fruit ripening process. The saponin content in berry of 4 years was relatively high at DAF 14 and 5 years specimens were at DAF 7. The most abundant ginsenoside Re in berry was converted into ginsenoside Rg2 by an enzyme bgp1 via mass production of recombinant enzyme bgp1. After bioconversion, the metabolites were checked for their cytotoxicity evaluation in human dermal fibroblast and murine melanoma B16BL6 skin cell lines. In vitro cell viability assay has shown that it was non-toxic to human dermal fibroblast (HDF) and murine melanoma B16BL6 (B16) cell lines. However, fermented green ginseng berry (100 ug/ml) was found to be toxic to B16. The present results clearly suggested that the bio converted green ginseng berry was found to be more effective in suppressing melanin content and enhancing whitening activity in HDF and BL6BL1.

[1] Md. Amdadul Huq a, Yeon-Ju Kimb, Fayeza Md. Siraj a, Deok-Chun Yang a Biotechnology and Applied Biochemistry 2016;63:532 – 538.

[2] Yan Jin, Yeon-Ju Kim*, Ji-Na Jeon, Chao Wang, Jin-Woo Min, Hae-Yong Noh, Deok-Chun Yang* Plant Foods for Human Nutrition.2015;70:141 – 145.