Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608154
Poster Session
Georg Thieme Verlag KG Stuttgart · New York

Bioactive potential and role of secondary metabolites within the microorganism community of the sea grass Posidonia oceanica

A Alfattani
1   School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, Geneva, Switzerland, Geneva, Switzerland
,
E Blanchet
2   Sorbonne Universités, UPMC Univ Paris 06, CNRS, Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM), Observatoire Océanologique, Banyuls-sur-Mer, France, Banyuls-sur-Mer, France
,
J Oliveira Da Silva
2   Sorbonne Universités, UPMC Univ Paris 06, CNRS, Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM), Observatoire Océanologique, Banyuls-sur-Mer, France, Banyuls-sur-Mer, France
,
S Leoni
3   Microbiology Unit, Department of Botany and Plant Biology, University of Geneva, CH-1211 Geneva, Switzerland, Geneva, Switzerland
,
PM Allard
1   School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, Geneva, Switzerland, Geneva, Switzerland
,
E Queiroz
1   School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, Geneva, Switzerland, Geneva, Switzerland
,
M Roy
4   Université Toulouse 3 Paul Sabatier, CNRS, ENFA, UMR5174 EDB (Laboratoire Évolution & Diversité Biologique), 118 route de Narbonne, 31062 Toulouse, France, Toulouse, France
,
J Chave
4   Université Toulouse 3 Paul Sabatier, CNRS, ENFA, UMR5174 EDB (Laboratoire Évolution & Diversité Biologique), 118 route de Narbonne, 31062 Toulouse, France, Toulouse, France
,
R Lami
2   Sorbonne Universités, UPMC Univ Paris 06, CNRS, Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM), Observatoire Océanologique, Banyuls-sur-Mer, France, Banyuls-sur-Mer, France
,
K Perron
3   Microbiology Unit, Department of Botany and Plant Biology, University of Geneva, CH-1211 Geneva, Switzerland, Geneva, Switzerland
,
D Stien
2   Sorbonne Universités, UPMC Univ Paris 06, CNRS, Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM), Observatoire Océanologique, Banyuls-sur-Mer, France, Banyuls-sur-Mer, France
,
JL Wolfender
1   School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, Geneva, Switzerland, Geneva, Switzerland
› Author Affiliations
Further Information

Publication History

Publication Date:
24 October 2017 (online)

 

Posidonia oceanica a dominant herb of marine grassland from the Mediterranean has been selected for this project as a unique model system for studying its microbiota. This species is known for its longevity and is potentially the host for a large endophyte community (1). The aim of the project was to comprehensively profile the endophyte community of this peculiar marine plant, use it as a model for deciphering microbiome interactions and investigate if this can inspire the discovery of new antibacterial agents.

The diversity of endophytic microbial communities from leaves and rhizomes was assessed by high throughput sequencing approaches and 23 strains of fungi and bacteria were isolated and identified. These strains were cultivated and extracted by ethyl acetate for metabolite profiling and antimicrobial activity screening.

All samples were dereplicated by UHPLC-HRMS/MS and molecular networking for a preliminary survey of their secondary metabolite composition. Surprisingly some of the screened species presented unexpected high amounts of fatty acids. Among all fungal strains Lulwoana sp. were found very specific to the marine environment. Their metabolite profiles reveal the presence of original corymbiferan lactones and their targeted isolation reveal the presence of a new methylated derivative. Extensive profiling in different cultivation media also demonstrated the diversity of such compounds in these endophytes.

In parallel the extracts were screened for their antimicrobial activity against Staphylococcus aureus, Propionibacterium acnes and Pseudomonas aeruginosa. Penicillium and Fusarium spp. strains were found to display interesting antimicrobial activities and a correlation between their chemical profiles and activities will be presented.

All the results obtained provide a first survey of chemical and bioactivity profiles in view of studying interactions between the members of this unique community.

This work has benefited from a SECIL grant from ANR (15-CE21 – 0016) and SNF (310030E-164289/1).

Vohník M, et al. Mycorrhiza. 2015;25:663 – 72.