CC BY-NC-ND 4.0 · Rev Bras Ginecol Obstet 2017; 39(10): 541-544
DOI: 10.1055/s-0037-1606349
Original Article
Thieme Revinter Publicações Ltda Rio de Janeiro, Brazil

Sirtuin 1 Levels in Recurrent Implantation Failure

Níveis de sirtuína 1 em falhas recorrentes de implantação
Yaprak Engin-Ustun
1   Reproductive Endocrinology Department, University of Health Sciences, Dr. Zekai Tahir Burak Health Practice Research Center, Ankara, Turkey
,
A. Seval Ozgu-Erdinc
1   Reproductive Endocrinology Department, University of Health Sciences, Dr. Zekai Tahir Burak Health Practice Research Center, Ankara, Turkey
,
Emel Kiyak Caglayan
1   Reproductive Endocrinology Department, University of Health Sciences, Dr. Zekai Tahir Burak Health Practice Research Center, Ankara, Turkey
,
Cavidan Gulerman
1   Reproductive Endocrinology Department, University of Health Sciences, Dr. Zekai Tahir Burak Health Practice Research Center, Ankara, Turkey
,
Esma Sarikaya
1   Reproductive Endocrinology Department, University of Health Sciences, Dr. Zekai Tahir Burak Health Practice Research Center, Ankara, Turkey
,
Ayla Aktulay
1   Reproductive Endocrinology Department, University of Health Sciences, Dr. Zekai Tahir Burak Health Practice Research Center, Ankara, Turkey
,
Canan Demirtas
2   Biochemistry Department, Gazi University, Ankara, Turkey
,
Salim Erkaya
1   Reproductive Endocrinology Department, University of Health Sciences, Dr. Zekai Tahir Burak Health Practice Research Center, Ankara, Turkey
,
Nafiye Yilmaz
1   Reproductive Endocrinology Department, University of Health Sciences, Dr. Zekai Tahir Burak Health Practice Research Center, Ankara, Turkey
› Author Affiliations
Further Information

Publication History

13 February 2017

03 August 2017

Publication Date:
01 September 2017 (online)

Abstract

Sirtuin 1 has an important role in cellular processes, including apoptosis and cellular stress. The purpose of this study was to assess serum sirtuin 1 levels in women with recurrent implantation failure (RIF). In this cross-sectional study, we included 28 women with RIF, 29 healthy women who had conceived by in vitro fertilization (IVF), and 30 women with a 1-cycle failure of IVF as controls. Human serum nicotinamide adenine dinucleotide (NAD)-dependent deacetylase sirtuin-1 (SIRT1/SIRT2L1) levels were detected using a commercial colorimetric kit. Recurrent implantation failure patients have higher sirtuin 1 levels than non-pregnant women and healthy pregnant women, but this difference did not reach statistical significance due to the low number of patients in our study. These higher sirtuin 1 levels may result from the inflammation imbalance of RIF patients. The only statistically significant correlation found was between age and sirtuin (r = 0.277, p = 0.009).

Resumo

A sirtuína 1 tem importante função nos processos celulares, incluindo a apoptose e o estresse celular. O objetivo deste estudo é o de avaliar níveis de sirtuína 1 em mulheres com falhas recorrentes de implantação (FRI). Neste estudo cruzado, incluímos 28 mulheres com FRI, 29 mulheres saudáveis que deram à luz por fertilização in vitro (FIV) bem-sucedida, e 30 mulheres com 1 ciclo de FIV malsucedido como controle. Os níveis de sirtuína 1 em soro humano de desacetilase dependente de dinucleotídeo de nicotinamida adenina (DNA) (SIRT1/SIRT2L1) foram detectados usando um kit colorimétrico comercial. Pacientes com FRI tiveram níveis de sirtuína 1 superiores às pacientes grávidas e aos controles, mas esta diferença não atingiu significância estatística devido ao baixo número de pacientes envolvidos. Estes níveis mais altos de sirtuína 1 podem ser resultado da inflamação desigual em pacientes com FRI. A única correlação estatisticamente significante encontrada foi entre idade e sirtuína (r = 0,277, p = 0,009).

 
  • References

  • 1 Simon A, Laufer N. Assessment and treatment of repeated implantation failure (RIF). J Assist Reprod Genet 2012; 29 (11) 1227-1239
  • 2 Margalioth EJ, Ben-Chetrit A, Gal M, Eldar-Geva T. Investigation and treatment of repeated implantation failure following IVF-ET. Hum Reprod 2006; 21 (12) 3036-3043
  • 3 Liang PY, Diao LH, Huang CY. , et al. The pro-inflammatory and anti-inflammatory cytokine profile in peripheral blood of women with recurrent implantation failure. Reprod Biomed Online 2015; 31 (06) 823-826
  • 4 Hekimoğlu R, Pergin A, Uğur Y, Beksaç S, Turğal M, Çakar N. Impaired implantation and hereditary thrombophila; expression of LIF (Leukemia Inhibitory Factor) on extravillous trophoblasts. Gynecol Obstet Reprod Med. 2012; 18: 123-126
  • 5 Orsi NM, Tribe RM. Cytokine networks and the regulation of uterine function in pregnancy and parturition. J Neuroendocrinol 2008; 20 (04) 462-469
  • 6 Haigis MC, Guarente LP. Mammalian sirtuins--emerging roles in physiology, aging, and calorie restriction. Genes Dev 2006; 20 (21) 2913-2921
  • 7 Rodgers JT, Puigserver P. Fasting-dependent glucose and lipid metabolic response through hepatic sirtuin 1. Proc Natl Acad Sci U S A 2007; 104 (31) 12861-12866
  • 8 Zhang J, Fang L, Lu Z. , et al. Are sirtuins markers of ovarian aging?. Gene 2016; 575 (2 Pt 3): 680-686
  • 9 Nogueiras R, Habegger KM, Chaudhary N. , et al. Sirtuin 1 and sirtuin 3: physiological modulators of metabolism. Physiol Rev 2012; 92 (03) 1479-1514
  • 10 Schug TT, Xu Q, Gao H. , et al. Myeloid deletion of SIRT1 induces inflammatory signaling in response to environmental stress. Mol Cell Biol 2010; 30 (19) 4712-4721
  • 11 Kiyak Caglayan E, Engin-Ustun Y, Gocmen AY, Polat MF, Aktulay A. Serum sirtuin 1 levels in patients with polycystic ovary syndrome. J Obstet Gynaecol 2015; 35 (06) 608-611
  • 12 World Medical Association. World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects. JAMA 2013; 310 (20) 2191-2194
  • 13 Luz CM, Giorgi VS, Coelho Neto MA, Martins WP, Ferriani RA, Navarro PA. Association between number of formed embryos, embryo morphology and clinical pregnancy rate after intracytoplasmic sperm injection. Rev Bras Ginecol Obstet 2016; 38 (09) 465-470
  • 14 Laird SM, Tuckerman EM, Li TC. Cytokine expression in the endometrium of women with implantation failure and recurrent miscarriage. Reprod Biomed Online 2006; 13 (01) 13-23
  • 15 Fukui A, Funamizu A, Yokota M. , et al. Uterine and circulating natural killer cells and their roles in women with recurrent pregnancy loss, implantation failure and preeclampsia. J Reprod Immunol 2011; 90 (01) 105-110
  • 16 Saini V, Arora S, Yadav A, Bhattacharjee J. Cytokines in recurrent pregnancy loss. Clin Chim Acta 2011; 412 (9-10): 702-708
  • 17 Boeddeker SJ, Hess AP. The role of apoptosis in human embryo implantation. J Reprod Immunol 2015; 108: 114-122
  • 18 Shirane A, Wada-Hiraike O, Tanikawa M. , et al. Regulation of SIRT1 determines initial step of endometrial receptivity by controlling E-cadherin expression. Biochem Biophys Res Commun 2012; 424 (03) 604-610
  • 19 Liu-Bryan R. Inflammation and intracellular metabolism: new targets in OA. Osteoarthritis Cartilage 2015; 23 (11) 1835-1842
  • 20 Conti V, Corbi G, Manzo V, Pelaia G, Filippelli A, Vatrella A. Sirtuin 1 and aging theory for chronic obstructive pulmonary disease. Anal Cell Pathol (Amst) 2015; 2015: 897327
  • 21 Van Gool F, Gallí M, Gueydan C. , et al. Intracellular NAD levels regulate tumor necrosis factor protein synthesis in a sirtuin-dependent manner. Nat Med 2009; 15 (02) 206-210
  • 22 Kong S, Yeung P, Fang D. The class III histone deacetylase sirtuin 1 in immune suppression and its therapeutic potential in rheumatoid arthritis. J Genet Genomics 2013; 40 (07) 347-354
  • 23 Lugrin J, Ciarlo E, Santos A. , et al. The sirtuin inhibitor cambinol impairs MAPK signaling, inhibits inflammatory and innate immune responses and protects from septic shock. Biochim Biophys Acta 2013; 1833 (06) 1498-1510