In Situ Endothelialization Promoted by SEMA4D and CXCL12 for Titanium-Based Biomaterials

Yuanyuan Cui; Feng Zhou; Lai Wei; Qiang Song; Jianying Tan; Zheng Zeng; Junying Chen; Nan Huang

doi:10.1055/s-0037-1605569

Seminars in Thrombosis and Hemostasis, Table of Contents

Semin Thromb Hemost 2018; 44(01): 070-080
DOI: 10.1055/s-0037-1605569

Review Article

Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

In Situ Endothelialization Promoted by SEMA4D and CXCL12 for Titanium-Based Biomaterials

Yuanyuan Cui

¹Key Laboratory of Advanced Technology of Materials, Southwest Jiaotong University, Chengdu, People's Republic of China

,

Feng Zhou

¹Key Laboratory of Advanced Technology of Materials, Southwest Jiaotong University, Chengdu, People's Republic of China

²Institute of Aeronautics and Astronautics, University of Electronic Science and Technology of China, Chengdu, People's Republic of China

,

Lai Wei

¹Key Laboratory of Advanced Technology of Materials, Southwest Jiaotong University, Chengdu, People's Republic of China

,

Qiang Song

¹Key Laboratory of Advanced Technology of Materials, Southwest Jiaotong University, Chengdu, People's Republic of China

,

Jianying Tan

¹Key Laboratory of Advanced Technology of Materials, Southwest Jiaotong University, Chengdu, People's Republic of China

,

Zheng Zeng

¹Key Laboratory of Advanced Technology of Materials, Southwest Jiaotong University, Chengdu, People's Republic of China

,

Junying Chen

¹Key Laboratory of Advanced Technology of Materials, Southwest Jiaotong University, Chengdu, People's Republic of China

,

Nan Huang

¹Key Laboratory of Advanced Technology of Materials, Southwest Jiaotong University, Chengdu, People's Republic of China

› Author Affiliations

Abstract

In situ endothelialization, aiming to create implantation surfaces capable of self-endothelialization, seems to be an extremely promising solution, particularly on those blood-contacting surfaces. In this research study, we immobilized the soluble semaphorin 4D (SEMA4D) and C-X-C motif chemokine ligand 12 (CXCL12) biomolecules together with heparin onto the metal-based biomaterial surfaces to achieve in situ endothelialization of modified samples both by stimulating the neighboring endothelial cells (ECs) migration and by capturing the circulating endothelial progenitor cells (EPCs) directly from the blood circulation. X-ray photoelectron spectroscopy data demonstrate the successful immobilization of SEMA4D and CXCL12. Due to the presence of heparin, hemocompatibility was also improved after modification. Although EC migration was mainly mediated by SEMA4D with the coordination of CXCL12, EC proliferation and haptotaxis property were also enhanced, while EC chemotaxis was slightly suppressed because the further immobilization of CXCL12 influences the release of SEMA4D. The results of the ex vivo EPC capturing assay indicated the mobilization of CXCL12 promotes EPC adhesion. In vivo implantation further demonstrated that CXCL12 cooperates with SEMA4D to promote a process of in situ endothelialization.

Keywords

in situ endothelialization - endothelial cell migration - endothelial progenitor cell - SEMA4D - CXCL12

Full Text

References

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