J Pediatr Infect Dis 2017; 12(04): 249-255
DOI: 10.1055/s-0037-1603587
Review Article
Georg Thieme Verlag KG Stuttgart · New York

Malaria in Children: Diagnostic Tools in Resource-Limited Settings

Narong Jaturas1, 2, Tooba Mahboob1, Nadiah Syafiqah Binti Nor Azman3, Tan Tian-Chye1, Veeranoot Nissapatorn1, 4
  • 1Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
  • 2Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand
  • 3School of Pharmacy, University of Nottingham Malaysia, Jalan, Semenyih, Kuala Lumpur, Malaysia
  • 4School of Allied Health Sciences, Walailak University, Thailand
Further Information

Publication History

26 December 2016

23 January 2017

Publication Date:
08 June 2017 (eFirst)

Abstract

Malaria contributes to a major reason for the cause of fatality and serious health problems among children mainly in various parts of tropical Africa and Asia. The extraction of DNA plays a vital role in the diagnosis of this life-threatening parasite Plasmodium. Difficulties with existing extraction methods of DNA are increasing with time including detection limits, time duration, sensitivity, availability of chemicals, and cost. Consequently, there is now renewed interest in finding of novel methods for DNA extraction of Plasmodium. However, the extraction of DNA by using microwave irradiation is found to be most convenient and suitable due to its low-cost and usage of common chemical reagents especially for the children in poor resource settings. In addition, the low cost, noninvasive malaria diagnostics tests are needed to carry out particularly for children to avoid accidental infections which may happen due to drawing blood.