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J Pediatr Infect Dis 2017; 12(04): 256-263
DOI: 10.1055/s-0037-1603561
Review Article
Georg Thieme Verlag KG Stuttgart · New York

Prospects on the Application of DNA Barcoding on Soil-Transmitted Helminths in Children

Guo-Jie Brandon-Mong
1   Department of Parasitology, Faculty of Medicine Building, University of Malaya, Kuala Lumpur, Malaysia
,
Romano Ngui
1   Department of Parasitology, Faculty of Medicine Building, University of Malaya, Kuala Lumpur, Malaysia
,
Veeranoot Nissapatorn
1   Department of Parasitology, Faculty of Medicine Building, University of Malaya, Kuala Lumpur, Malaysia
2   School of Allied Health Sciences, Walailak University, Thailand
› Institutsangaben
Weitere Informationen

Publikationsverlauf

20. Dezember 2016

10. Januar 2017

Publikationsdatum:
08. Juni 2017 (online)

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Abstract

Since more than a decade, DNA barcoding has been widely used to examine biological samples and differentiate species, as well as employed in ecological and conservational studies. There is a growing interest of DNA barcoding, particularly in medical parasitology, but its potential utility in soil-transmitted helminths (STHs) remains unclear. Therefore, in this article, we review the studies using DNA barcoding and its applications in medical parasitology with special focus on STHs such as Ascaris lumbricoides, Ancylostoma duodenale, Necator americanus, and Trichuris trichiura. DNA barcoding is reliable for identifying STH species as well as its cryptic species. In addition, epidemiological data and the impacts of STH infections on children are discussed. This article further discusses the paucity of STH DNA barcodes (partial cytochrome oxidase subunit 1 [COI] mitochondrial DNA [mtDNA] sequences) in two gene banks; National Center for Biotechnology Information (NCBI) and Barcode of Life Data (BOLD) Systems. It also highlights the future prospects of DNA barcoding including primer designing and reference library on STHs.

Keywords

children - cryptic species - DNA barcoding - PCR - soil-transmitted helminths
 

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