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DOI: 10.1055/s-0036-1596532
Investigations in flavonoid content of Camelina sativa (L.) Crantz during seed development
Publication History
Publication Date:
14 December 2016 (online)
In recent years, Camelina sativa (L.) Crantz has known a renewed interest for its agronomic potential. This oilseed crop has a short development cycle (85 – 100 days) and is naturally tolerant to biotic and abiotic stresses [1]. This Brassicaceae is undemanding in fertilizer input and requires less pesticide compared to traditional oilseed crops [2]. Currently this plant is mainly grown for its seeds with unusual fatty acid profile [3] and phytochemical investigations of the seeds are still emerging. In order to complete the phytochemical characterization of Camelina, the flavonoid contents of seeds has been extracted by the successive contribution of 3 solvents (MeOH, CHCl3, H2O). The hydroalcoholic fraction was purified by HPLC and collected products were analyzed by LC-MS and NMR to be identified. This method allowed the characterization of two novel flavonoids in seeds of Camelina: quercetin-5b-O-sinapyl-2"-O-apiosyl-3-O-rutinoside and epicatechin-7-O-glucose.
These 2 compounds have a contrasted dynamic accumulation during seed development. The quercetin-5b-O-sinapyl-2"-O-apiosyl-3-O-rutinoside accumulates essentially between 15 and 25 days after flowering (DAF). Thus, the area ratio was 0.1, 0.4 and 0.5 at 15, 25 and 35 DAF, respectively. In contrast, the epicatechin-7-O-glucose accumulates essentially between 25 and 35 DAF. This compound was not detectable at 15 and 25 DAF and the area ratio was 3.7 at 35 DAF.
To conclude, this study allowed to characterize two novel flavonoids in seeds of Camelina and to describe their accumulation during seed development.
Keywords: Camelina sativa, seed, quercetin-5b-O-sinapyl-2"-O-apiosyl-3-O-rutinoside, epicatechin-7-O-glucose, LC-MS, NMR.
References:
[1] Gugel RK, Falk KC. Agronomic and seed quality evaluation of Camelina sativa in western Canada. Can J Plant Sci 2006; 86: 1047 – 1058
[2] Moser BR. Camelina (Camelina sativa L.) oil as a biofuels feedstock: Golden opportunity or false hope? Lipid Technol 2010; 22: 270 – 273
[3] Vollmann J, Moritz T, Kargl C, Baumgartner S, Wagentristl H. Agronomic evaluation of camelina genotypes selected for seed quality characteristics. Ind Crops Prod 2007; 26: 270 – 277