Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596494
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

HPLC-DAD-MS analysis of extracts from flowers, leaves, fruits and branches of Ligustrum vulgare and their effect on cytokines secretion by human neutrophils

ME Czerwińska
1   Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02 – 097 Warsaw, Poland
,
M Warowny
1   Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02 – 097 Warsaw, Poland
,
AK Kiss
1   Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02 – 097 Warsaw, Poland
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 

Ligustrum vulgare L. (Oleaceae) is a shrub commonly found in Europe and Asia. The extracts from its leaves and flowers were traditionally used in oropharyngeal inflammations, aphthae and chronic tonsillitis. Fruits served as mildly laxative agent [1].

The aim of the study was a comparison of the composition of aqueous and ethanolic extracts from leaves, flowers, fruits and branches of L. vulgare using HPLC-DAD-MS/MS method, as well as determination of total phenolic content (TPC) in the extracts. In order to compare their biological activity the model of human neutrophils, which are cells of immune system involved in the inflammation, was used. The effect of extracts on cytokines, such as IL-8 and TNFα, secretion by neutrophils was determined.

HPLC-DAD-MS/MS analysis was performed on a Zorbax SB C18 column. A linear gradient system of mobile phase A (water/acetonitrile/formic acid, 95:5:0.1, v/v/v) and mobile phase B (methanol) was used: 0 – 60 min., 1 – 60% B. The Folin-Ciocalteu method was used to determine TPC, expressed as gallic acid equivalents. The effect of extracts on IL-8 and TNFα secretion after LPS-induced stimulation was established by ELISA assay.

The most abundant compound of leaves, flowers and branches extracts was oleuropein, whereas the major compound of fruits was nuezhenide. The highest TPC was observed in branches ethanolic (148.9 ± 3.2 mg/g) and aqueous (137.0 ± 2.9 mg/g) extracts. These extracts were also the most effective inhibitors of IL-8 and TNFα secretion. The branches aqueous extract (25 µg/ml) inhibited IL-8 secretion by 23.1 ± 4.8%, whereas ethanolic extract (100 µg/ml) inhibited TNFα release by 37.3 ± 16.4% compared with control. In conclusion, the extracts from different parts of L. vulgare constitute a potential source of biologically active compounds, and the inhibition of cytokines secretion might partially justify anti-inflammatory effectiveness of this plant material preparations in the treatment of disorders in folk medicine.

Keywords: Ligustrum vulgare, oleuropein, secoiridoids, neutrophils.

References:

[1] Fournier P. Encyclopédie biologique, Plantes médicinales et vénéneuses de France. Paris: Paul Lechevalier; 1948