Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596480
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Chemical characterization and in vitro antibacterial activity of Kalanchoe brasiliensis Cambess (Crassulaceae)

OAS Mayorga
1   Graduate Program in Pharmaceutical Sciences, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, Campus Universitário, São Pedro, CEP 36036 – 900, Juiz de Fora, Minas Gerais, Brazil
,
JR Florencio
1   Graduate Program in Pharmaceutical Sciences, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, Campus Universitário, São Pedro, CEP 36036 – 900, Juiz de Fora, Minas Gerais, Brazil
,
JDG de Santana
1   Graduate Program in Pharmaceutical Sciences, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, Campus Universitário, São Pedro, CEP 36036 – 900, Juiz de Fora, Minas Gerais, Brazil
,
C Feres-Netto
2   Pharmacy Course, Faculty of Pharmacy, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, Campus Universitário, São Pedro, CEP 36036 – 900, Juiz de Fora, Brazil
,
DM Pedrosa
2   Pharmacy Course, Faculty of Pharmacy, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, Campus Universitário, São Pedro, CEP 36036 – 900, Juiz de Fora, Brazil
,
YFG Costa
2   Pharmacy Course, Faculty of Pharmacy, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, Campus Universitário, São Pedro, CEP 36036 – 900, Juiz de Fora, Brazil
,
NCC Pinto
3   Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, 36036 – 900, Juiz de Fora, MG, Brazil
,
E Scio
3   Laboratory of Bioactive Natural Products, Department of Biochemistry, Federal University of Juiz de Fora, 36036 – 900, Juiz de Fora, MG, Brazil
,
OV Sousa
4   Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, Campus Universitário, São Pedro, CEP 36036 – 900, Juiz de Fora, Minas Gerais, Brazil
,
MS Alves
4   Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n, Campus Universitário, São Pedro, CEP 36036 – 900, Juiz de Fora, Minas Gerais, Brazil
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 

Kalanchoe brasiliensis Cambess (Crassulaceae), popularly known as "Saião", is a Brazilian medicinal plant distributed from Bahia to São Paulo and traditionally used to treat injuries, abscesses, enlarged ganglia and inflammatory processes [1,2]. The current study aimed to characterize the chemical composition of the ethanolic extract 50% (v/v) obtained from K. brasiliensis leaves collected before blooming using HPLC-DAD [3] and to investigate the antibacterial activity of this extract at different concentrations (30%, 50% and 70%). The chromatography profile and the UV spectrum analysis suggested the significant presence of flavonoids. The antibacterial activity was established according to the Clinical Laboratory Standards Institute (CLSI) guidelines by the Minimal Inhibitory Concentration (MIC) [4] using the microdilution method followed by the Minimal Bactericidal Concentration (MBC), which allows to classify the antibacterial effect as bacteriostatic or bactericidal [5]. Staphylococcus aureus subsp. aureus (ATCC® 29213™), Staphylococcus aureus subsp. aureus (ATCC® 6538™), Pseudomonas aeruginosa (ATCC® 9027™), Pseudomonas aeruginosa (ATCC® 27853™), Escherichia coli (ATCC®25922™), Escherichia coli (ATCC®10536™), Salmonella enterica subsp. enterica serovar Typhimurium (ATCC® 13311™) and Salmonella enterica subsp. enterica serovar Choleraesuis (ATCC® 10708™) were used as reference strains. The ethanolic extract at 30% and 50% demonstrated bacteriostatic effect against S. Typhimurium ATCC® 13311™ and S. Choleraesuis ATCC® 10708™, with MIC values of 5000 µg/mL. Additionally, the ethanolic extract at 70% revealed bacteriostatic effect against S. aureus ATCC® 6538™ and ATCC®25922™ and against S. Thyphimurium ATCC® 13311™ and S. Choleraesuis ATCC® 10708™, with MIC values of 5000 µg/mL, showing a broad spectrum of action. These results suggest that K. brasiliensis can be an interesting source of bioactive molecules with antibacterial potential.

Acknowledgements: This investigation was supported by UFJF, FAPEMIG, CAPES, OEA and GCUB.

Keywords: Kalanchoe brasiliensis, flavonoids, HPLC-DAD, anti-bacterial agents, medicinal plants.

References:

[1] Costa SS, Jossang A, Bodo B, Souza ML, Moraes VL. Patuletin acetylrhamnosides from Kalanchoe brasiliensis as inhibitors of human lymphocyte proliferative activity. J Nat Prod 1994; 57: 1503 – 1510

[2] Mourão RH, Santos FO, Franzotti EM, Moreno MP, Antoniolli AR. Antiinflammatory activity and acute toxicity (LD50) of the juice of Kalanchoe brasiliensis (Camb.) leaves picked before and during blooming. Phytother Res 1999; 13: 352 – 354.

[3] Costa ACO, Fernandes JM, Themístocles NNS, Mendonça JN, Tomaz JC, Lopes NP, Alberto L, Soares L, Zucolotto SM. Quantification of chemical marker of Kalanchoe brasiliensis (Crassulaceae) leaves by HPLC-DAD. J Liq Chromatogr Rel Technol 2015, 38: 795 – 800

[4] Clinical and Laboratory Standards Institute. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard – Ninth Edition (M07-A9). Wayne: CLSI, 2012

[5] Andrews JM. Determination of minimum inhibitory concentrations. J Antimicrob Chemother 2001; 48: 5 – 16