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DOI: 10.1055/s-0036-1596476
Isolation and determination of antioxidant capacities of anthocyanin from Berberis integerrima Bunge fruits
Publication History
Publication Date:
14 December 2016 (online)
Anthocyanins are flavonoid pigments in plants and their products [1]. Reported advantages of anthocyanin comprise anti-inflammatory, anti-oedema activities and anti-tumor activity [2].
Berberis integerrima Bunge belongs to Berberidaceae which is used as therapeutic agent in gastrointestinal diseases, bleeding, fever, malaria and hepatitis [3].
In this study, freeze-dried fruits of Berberis integerrima Bunge were extracted by ethanol. Crude extract was partitioned between ethyl acetate and 0.3 TFA%. The aquatic fraction was loaded on glass column containing amberlite XAD-7 as a stationary phase. Fraction eluted with %0.3TFA in methanol and was collected as a rich source of anthocyanin and analyzed by HPLC-UV. The result indicated an anthocyanin with purity of 91% (AFBI).
PH-differential method showed anthocyanin content is14.36 ± 0.33 mg/g before purification by XAD-7and 34.51 ± 0.42 mg/g in AFBI.The antioxidant activity of AFBIF measured by different methodswhich is demonstrated in Table 1. Also, Trolox equivalent value measured by ORAC assay is 0.8 ± 0.11 and nitric oxide scavenging assay of 200 µg/ml is 97.04 ± 0.69%. Results indicated that Berberries integrimma Bunge fruits are rich sources of anthocyanin and have strong capacity to scavenge different radicals. In future studies, it can be considered in prevention of free radical damages.
Method |
IC50 for AFBIF (µg/ml) |
IC50 for Quercetin (µg/ml) |
DPPH assay |
66 ± 0.04 |
26.51 ± 0.06 |
FRAP assay |
11 ± 0.16 |
8.69 ± 0.03 |
ABTS |
60 ± 2.1 µg |
25.64 ± 0.02 |
Keywords: Anthocyanin, Berberis integerrima Bunge, antioxidant.
References:
[1] Diaconeasa Z. Leopold L. Rugină D. Ayvaz H. Socaciu C. Antiproliferative and antioxidant properties of anthocyanin rich extracts from Blueberry and Blackcurrant juice. Int JMol Sci 2015;16: 2352 – 2365
[2] Kong J-M. Chia L-S. Goh N-K. Chia T-F. Brouillard R. Analysis and biological activities of anthocyanins. Phytochem 2003; 64: 923 – 933
[3] Ashraf H. Heidari R. Nejati V. Ilkhanipoor M. Effects of aqueous extract of Berberis integerrimaroot on some physiological parameters in Streptozotocin-Induced diabetic rats. Iran J Pharm Res 2013;12: 425 – 434