Subscribe to RSS
DOI: 10.1055/s-0036-1596438
Anti-diabetic activity of flavonoids isolated from the aerial parts of Platycodon grandiflorum in zebrafish, a model of type 2 diabetes
Publication History
Publication Date:
14 December 2016 (online)
Platycodon grandiflorum A. DC. (family Campanulaceae) is a perennial herb and distributed in Korea, China, Russia and Japan [1]. The root of P. grandiflorum, a well-known traditional Chinese medicine and food reserves, has been used for treatment of cough, bronchitis, sore throat and purulent disease [2]. It is widely cultivated in Korea, and has been used more often for food than for medicinal purposes [2]. The zebrafish model for type 2 diabetes was induced by exposure to excess insulin [3]. In the course of a search for anti-diabetic compounds from natural products, we have found that the EtOH extract of aerial parts of P. grandiflorum has antihyperglycemic activity in the zebrafish model for type 2 diabetes. Eight flavone glycosides (1 – 8) and a phenolic compound (9) were isolated from the EtOAc and n-BuOH fractions of the EtOH extract. The structures of isolated compounds were determined as luteolin 7-O-(6″-O-acetyl) neohesperidoside (1) and apigenin 7-O-(6″-O-acetyl) neohesperidoside (2), luteolin 7-O-neohesperidoside (3), apigenin 7-O-neohesperidoside (4), luteolin 7-O-(6″-O-acetyl) glucoside (5), luteolin 7-O-glucoside (6), apigenin 7-O-glucoside (7), luteolin (8), and chlorogenic acid (9) from spectral data. Anti-diabetic activity of isolated compounds was evaluated by using a zebrafish model. Following insulin treatment, pancreatic islet size and fluorescence intensity were measured. Among isolated compounds, 2, 4, 6 and 8 revealed potent anti-diabetic activity for type 2 at a concentration of 1µM.
Keywords: Platycodon grandiflorum, Campanulaceae, type 2 diabetes, zebrafish, flavonoid.
References:
[1] Lee WT. Colored standard illustrations of Korean plants. Seoul: Academy Book 1996; 342
[2] Zhang L, Wang Y, Yang D, Zhang C, Zhang N, Li M, Liu Y. Platycodon grandiflorus - an ethnopharmacological, phytochemical andpharmacological review. J Ethanopharmacol 2015; 164: 147 – 161
[3] Yang X, Mei S, Gu H, Guo H, Zha L, Cai J, Li X, Liu Z, Cao W. Exposure to excess insulin (glargine) induces type 2 diabetes mellitus in mice fed on a chow diet. J Endocrinol 2014; 221: 469 – 480