Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596403
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

New Polish species with isolated Pulsatilla saponin D

G Łaska
1   Department of Environmental Protection and Management, Bialystok University of Technology, Wiejska 45A, 15 – 351 Białystok, Poland
,
A Sienkiewicz
1   Department of Environmental Protection and Management, Bialystok University of Technology, Wiejska 45A, 15 – 351 Białystok, Poland
,
A Piotrowska-Niczyporuk
2   Department of Plant Biochemistry and Toxicology, University of Bialystok, Ciolkowskiego 1J, 15 – 245 Białystok, Poland
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 

Plant species of the Pulsatilla genus (Ranunculaceae) are used for centuries in traditional Chinese medicine. It had been reported that plants from the Pulsatilla genus contain ranunculin, anemonin, protoanemonin, triterpenes and saponins (9%), mainly the oleanane and lupane-type saponins [1]. In Poland until now, has not been isolated the saponin D in Pulsatilla patens subsp. patens (L.) Mill. and in this study is done for the first time. This is very important because Pulsatilla saponin D have demonstrated multiple biological properties including antifungal, antimicrobial and cytotoxic activities. Additionally, this natural product has potential beneficial effects as a chemopreventive agent for critical health conditions including cancer [2]. Treatment with saponin D resulted in inhibition of cell growth/proliferation, angiogenesis and induction of apoptosis in cancer [3]. HPLC analysis of the methanolic extract of the crude root of Pulsatilla patens (L.) Mill. revealed the presence of Pulsatilla saponin D [hederagenin 3-O-α-L-rhamnopyranosyl(1→2)-[β-D-glucopyranosyl(1→4)]-α-L-arabinopyranoside] (Fig. 1) (patent application pending). The structure of saponin D was determined by spectroscopic techniques. This compound is also produced from the root of several Pulsatilla species, including Pulsatilla koreana Nakai, Pulsatilla chinensis (Bunge) Regel and Pulsatilla cernua (Thunb.) Spreng. Pulsatilla saponin D isolated from the root of Pulsatilla koreana Nakai showed potent inhibition rate of tumor growth (IR, 82%) at the dose of 6.4 mg/kg on the BDF1 mice bearing LLC cells [1].

Zoom Image
Fig. 1: The structure of saponin D isolated from Pulsatilla patens subsp. patens (L.) Mill.

Keywords: Medicinal plant, saponin D, chromatographic analysis, biological activity.

References:

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[2] Hong SW, Jung KH, Lee HS, Son MK, Yan HH, Kang NS, Lee J, Hong SS. SB365, Pulsatilla saponin D, targets c-Met and exerts antiangiogenic and antitumor activities. Carcinogenesis 2013; 34: 2156 – 2169

[3] Son MK, Jung KH, Lee HS, Lee H, Kim SJ, Yan HH, Ryu YL, Hong SS. SB365, Pulsatilla saponin D suppresses proliferation and induces apoptosis of pancreatic cancer cells. Oncol Rep 2013; 30: 801 – 808