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DOI: 10.1055/s-0036-1596403
New Polish species with isolated Pulsatilla saponin D
Publication History
Publication Date:
14 December 2016 (online)
Plant species of the Pulsatilla genus (Ranunculaceae) are used for centuries in traditional Chinese medicine. It had been reported that plants from the Pulsatilla genus contain ranunculin, anemonin, protoanemonin, triterpenes and saponins (9%), mainly the oleanane and lupane-type saponins [1]. In Poland until now, has not been isolated the saponin D in Pulsatilla patens subsp. patens (L.) Mill. and in this study is done for the first time. This is very important because Pulsatilla saponin D have demonstrated multiple biological properties including antifungal, antimicrobial and cytotoxic activities. Additionally, this natural product has potential beneficial effects as a chemopreventive agent for critical health conditions including cancer [2]. Treatment with saponin D resulted in inhibition of cell growth/proliferation, angiogenesis and induction of apoptosis in cancer [3]. HPLC analysis of the methanolic extract of the crude root of Pulsatilla patens (L.) Mill. revealed the presence of Pulsatilla saponin D [hederagenin 3-O-α-L-rhamnopyranosyl(1→2)-[β-D-glucopyranosyl(1→4)]-α-L-arabinopyranoside] (Fig. 1) (patent application pending). The structure of saponin D was determined by spectroscopic techniques. This compound is also produced from the root of several Pulsatilla species, including Pulsatilla koreana Nakai, Pulsatilla chinensis (Bunge) Regel and Pulsatilla cernua (Thunb.) Spreng. Pulsatilla saponin D isolated from the root of Pulsatilla koreana Nakai showed potent inhibition rate of tumor growth (IR, 82%) at the dose of 6.4 mg/kg on the BDF1 mice bearing LLC cells [1].
Keywords: Medicinal plant, saponin D, chromatographic analysis, biological activity.
References:
[1] Kim Y, Bang SC, Lee JH, Ahn BZ. Pulsatilla Saponin D: the antitumor principle from Pulsatilla koreana. Arch Pharm Res 2004; 27: 915 – 918
[2] Hong SW, Jung KH, Lee HS, Son MK, Yan HH, Kang NS, Lee J, Hong SS. SB365, Pulsatilla saponin D, targets c-Met and exerts antiangiogenic and antitumor activities. Carcinogenesis 2013; 34: 2156 – 2169
[3] Son MK, Jung KH, Lee HS, Lee H, Kim SJ, Yan HH, Ryu YL, Hong SS. SB365, Pulsatilla saponin D suppresses proliferation and induces apoptosis of pancreatic cancer cells. Oncol Rep 2013; 30: 801 – 808