Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596380
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Chemical profile of Justicia gendarussa a medicinal plant used for male contraception

MM Mnatsakanyan
1   School of Pharmaceutical Sciences, Phytochemistry and Bioactive Natural Products, University of Geneva, University of Lausanne, 30, quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland
,
EF Queiroz
1   School of Pharmaceutical Sciences, Phytochemistry and Bioactive Natural Products, University of Geneva, University of Lausanne, 30, quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland
,
L Marcourt
1   School of Pharmaceutical Sciences, Phytochemistry and Bioactive Natural Products, University of Geneva, University of Lausanne, 30, quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland
,
B Prajogo
2   Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Airlangga, Jl. Dharmawangsa Dalam, Surabaya 60286, Indonesia
,
JL Wolfender
1   School of Pharmaceutical Sciences, Phytochemistry and Bioactive Natural Products, University of Geneva, University of Lausanne, 30, quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 

Justicia gendarussa Burm. F. (Acanthaceae) is a medicinal plant used to reduce male fertility among the Papuan tribes in Indonesia [1]. Traditional water decoction and the pills containing standardised extract used in clinical trials were analysed by HPLC-PDA-ESI-MS and UHPLC-TOF-HRMS for chemical profiling. Using this approach several 2-aminobenzyl derivatives and two flavonoids were dereplicated. UV and HRMS data also suggested the presence of an additional new 2-aminobenzyl derivative. In order to quantify these compounds in the various extracts and identify the new constituent, an efficient single step medium pressure liquid chromatography (MPLC-UV) was performed. The seven main constituents were isolated and as expected one a new 2-aminobenzyl derivative was identified it structure being confirmed by NMR, and HRMS. Isolated standards were used to develop an HPLC-UV quantification method. The 2-aminobenzyl derivative and flavonoid content of the traditional water decoction was compared with the standardised extract used in clinical trials. This revealed that pills used in clinical trials contain mainly the flavonoid gendarusin A, hypothesised active compound, and that the 2-aminobenzyl derivative were significantly removed by the standardisation process. Comparison of various J. gendarussa specimens collected in different regions of Indonesia was useful for the selection of plant material rich in gendarusin A.

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Acknowledgements: The authors are thankful to the Swiss Science Foundation for the financial support of this project (Grant 2013 – 9483 to M.M.).

Keywords: Justicia gendarussa, male fertility, UHPLC-TOF-HRMS, 2-aminobenzyl derivatives.

References:

[1] Handayani L. A male contraceptive pill consists of Gandarusa (Justicia gendarussa Burm. F). J Indonesian Med Assoc 2007; 57: 279 – 284