Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596333
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Potential anti-inflammatory activity of selected plants from Asteraceae family in in vitro models

B Michalak
1   Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Waraw, Banacha 1 Street, 02 – 097 Warsaw, Poland
,
ME Czerwińska
1   Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Waraw, Banacha 1 Street, 02 – 097 Warsaw, Poland
,
AK Kiss
1   Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Waraw, Banacha 1 Street, 02 – 097 Warsaw, Poland
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 

Plants from the Asteraceae family are traditionally used for treating inflammation-associated diseases, such as atherosclerosis, diabetes mellitus, bowel diseases (IBD; Crohn's disease and ulcerative colitis), asthma, chronic obstructive pulmonary disease (COPD), skin diseases and periodontal inflammation [1]. These diseases are closely associated with an excessive inflammatory response of stimulated neutrophils [2]. That is why selected extracts were examined for their effect on pro-inflammatory functions of stimulated neutrophils such as: reactive oxygen species (ROS), interleukin 8 (IL-8), tumor necrosis factor alpha (TNFα) and adhesion molecules.

The inhibition of ROS production by neutrophils was determined using luminol- dependent chemiluminescence. The effect on IL-8 and TNFα production by neutrophils was measured by an enzyme-linked immunosorbent assay. The expression of adhesion molecules CD11b and CD62L on neutrophils was analyzed with flow cytometry.

Some genera of the Asteraceae family such as: Inula, Helianthus, Eupatorium, Achillea, Acmella and Anacyclus were selected for preparing 60% ethanolic extracts. Extracts were shown to modulate lipopolysaccharide (LPS)-triggered production of IL-8 (at 25 – 100 µg/mL) and production of TNF-α (at 25 – 100 µg/mL). They were active towards f-MLP induced reactive oxygen species production (at 2.5 – 100 µg/mL). Moreover, some extracts were also shown to decrease the expression of integrin CD11b and prevent the shedding of selectin CD62L.

Our observations suggest that some extracts from Asteraceae family could play a potential role in the prevention of inflammation-associated diseases.

Keywords: Asteraceae, neutrophils, inflammation.

References:

[1] Bessada SMF, Barreira JCM, Oliveira MPP. Asteraceae species with most prominent bioactivity and their potential applications: A review. Ind Crop Prod 2015; 76: 604 – 615

[2] Amulic B, Cazalet C, Hayes GL, Metzler KD, Zychlinsky A. Neutrophil Function: From Mechanisms to Disease. Annu Rev Immunol 2012; 30: 459 – 489