Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596295
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Total content of affinin from Heliopsis longipes extracts by HPLC

V de la Rosa-Lugo
1   Centro de Investigaciones Químicas, IICBA, Universidad Autónoma del Estado de Morelos Avenida Universidad 1001, Col. Chamilpa, 62209 Cuernavaca, Morelos, México
,
MÁ Ramírez-Cisneros
1   Centro de Investigaciones Químicas, IICBA, Universidad Autónoma del Estado de Morelos Avenida Universidad 1001, Col. Chamilpa, 62209 Cuernavaca, Morelos, México
,
MY Rios
1   Centro de Investigaciones Químicas, IICBA, Universidad Autónoma del Estado de Morelos Avenida Universidad 1001, Col. Chamilpa, 62209 Cuernavaca, Morelos, México
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 

Heliopsis longipes (A. Gray) Blake (Asteraceae), commonly named in Mexico “chilcuague” or “chilcuan”, its roots are used as local analgesic and anesthetic to treat discomforts, mainly associated with toothache pain [1]. H. longipes includes compounds with demonstrated antinociceptive properties [2]. Affinin [N-isobutyl-2(E), 6(Z),8(E)-decatrienamide] is the major component of H. longipes, being responsible for these effects [3]. Its antinociceptive properties are related to GABA release, as well as to the activation of opioid, serotoninergic and nitric oxide pathways [4]. The aim of this work was to develop an HPLC-UV method for quantitative determination of affinin from Heliopsis longipes extracts. Acetonic extract from roots was obtained. Affinin (≤95% purity) was used as standard. Quantification was carried out using a Dionex Ultimate 3000 HPLC system (LPG-3400 SD pump, DAD-3000 detector) equipped with a Dionex Acclaim 120 column (C18, 4.6 × 250 mm, 5 µm). Chromatographic analyses were performed at room temperature (20 – 25 °C), at a flow rate of 1.2 mL/min. The mobile phase was a gradient consisting of 100% solvent A (water) to 100% B (methanol) for 10 min. This composition was maintained 5 min and then returned to original conditions (100% A). 20µL of the solution was injected directly into the HPLC system and affinin was detected at 229nm. This method can be used for rapid quantification (15 min) of affinin (0.05 to 0.5 mg/mL, r2= 0.95). Moreover, this work enables to correlate affinin content with antinociceptive activity of Heliopsis longipes.

Acknowlegements: This work was financially supported by CONACyT-México (Grant number 241044).

Keywords: Heliopsis longipes (A.Gray) S.F. Blake, affinin, antinociception, quantification, HPLC-UV method.

References:

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[4] Déciga M, Rios MY, Aguilar AB. Antinociceptive effect of Heliopsis longipes extract and affinin in mice. Planta Med 2010; 79: 665 – 670