Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596238
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

High throughput screening to investigate Brazilian Cerrado biome plant extract bank chemical diversity

LS Espindola
1   Laboratório de Farmacognosia, Universidade de Brasília, Brasília, Brazil
2   Molecular Targets Laboratory, National Cancer Institute/NIH, Frederick, MD, USA
,
RG Dusi
1   Laboratório de Farmacognosia, Universidade de Brasília, Brasília, Brazil
2   Molecular Targets Laboratory, National Cancer Institute/NIH, Frederick, MD, USA
,
KR Gustafson
2   Molecular Targets Laboratory, National Cancer Institute/NIH, Frederick, MD, USA
,
J McMahon
2   Molecular Targets Laboratory, National Cancer Institute/NIH, Frederick, MD, USA
,
JA Beutler
2   Molecular Targets Laboratory, National Cancer Institute/NIH, Frederick, MD, USA
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 

Plants have historically been a rich source of successful anticancer drugs. Research trends indicate that natural products will also be among the most important sources of new drugs in the future [1, 2]. In order to probe the chemical diversity and therapeutic utility of the Brazilian Cerrado plant extract library [3] of the University of Brasília, Brazil, a total of 702 extracts – produced from 95 species representing 40 plant families was screened by high throughput screening technologies on tumor cells [4]. Specific tests were performed on the following cell lines: colon (Colo205 and Km12), renal (A498 and U031), liver (HEP3B and SKHEP) and osteosarcoma (MG63 and MG63.3), aiming to select extracts for the isolation of promising compounds. None of the extracts inhibited growth of liver cancer cells. Dose-response tests were conducted with 44 extracts in colon, renal and osteosarcoma. Half of those demonstrated IC50 values between 1.3 and 20 µg/mL. Based on 1H NMR profiles and selective activity, 17 extracts were tested in the NCI-60 cell screen [4]. Our research supports conservation of the Cerrado against ever increasing anthropogenic activity by safeguarding biodiversity information for this biome hotspot, with the view to develop molecules capable of inhibiting the growth of cancer cells.

Acknowledgements: The authors wish to thank the following Brazilian agencies for their financial support: CNPq and FAPDF.

Keywords: Brazilian Cerrado biome, Plant extracts, cancer cells, NCI-60 cells.

References:

[1] Atanasov AG, Waltenberger B, Pferschy-Wenzig E-M, Linder T, Wawrosch C, Uhrin P, Temml V, Wang L, Schwaiger S, Heiss EH, Rollinger JM, Schuster D, Breuss JM, Bochkov V, Mihovilovic MD, Kopp B, Bauer R, Dirsch VM, Stuppneret H. Discovery and resupply of pharmacologically active plant-derived natural products: A review. Biotechnol Adv 2015; 33: 1582 – 614

[2] Harvey AL, Edrada-Ebel R, Quinn RJ. The re-emergence of natural products for drug discovery in the genomics era. Nat Rev Drug Discov 2015; 14: 111 – 129

[3] de Mesquita ML, de Paula JE, Pessoa C, de Moraes MO, Costa-Lotufo LV, Grougnet R, Michel S, Tillequin F, Espindola LS. Cytotoxic activity of Brazilian Cerrado plants used in traditional medicine against cancer cell lines. J Ethnopharmacol 2009; 123: 439 – 445

[4] Shoemaker RH. The NCI60 human tumour cell line anticancer drug screen. Nat Rev Cancer 2006; 6: 813 – 823