Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596230
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

LC-MS-based metabolomics study on the effects of light qualitieson Phalaenopsis

TH Liu
1   Department of Pharmacy, Taipei Medical University, 250 Wuxing Street, Taipei 110, Taiwan
,
JD Wu
1   Department of Pharmacy, Taipei Medical University, 250 Wuxing Street, Taipei 110, Taiwan
,
YCA Chang
2   Department of Horticulture and Landscape Architecture, National Taiwan University, 1 Roosevelt Road Sec. 4, Taipei 106, Taiwan
,
CK Lee
1   Department of Pharmacy, Taipei Medical University, 250 Wuxing Street, Taipei 110, Taiwan
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 

Phalaenopsis is well known for its beautiful, moth-like flowers, and has become an important ornamental crop in the world. The production has been notably increased in Germany, Japan, the Netherlands, Taiwan, and the United States [1]. Light is one of the most important factors influencing plant growth. Light not only acts as the source of energy, but also plays a regulatory role in plant growth and reproduction processes [2]. In this study, we applied LC-MS technique combined with SIMCA-P+ software to explore the effects of light qualities on Phalaenopsis and to find the significantly different compounds which changed under seven light qualities. Our data showed a tendency thatmany compounds were significantly changed. The compounds were tentatively identified by accurate mass measurement, MS/MS fragmentation and database search. It showed that metabolites of Phalaenopsis under different light environments were different. According to the metabolic analysis, there are significant differences between the molecular weight149.1204, 223.1210, 273.2670, 339.2536, 342.1175, 488.3149, and 791.5891. In addition to the molecular weight 223.1210 was identified as anhalonidine. It involved biosynthesis of alkaloids derived from shikimate pathway. Different strategies of light supplement might be applied to enhance Phalaenopsis growth for commercial production.

Acknowledgements: The authors are grateful to Ministry of Science and Technology, Taiwan for financial assistance (NSC 101 – 2320-B-038 – 013-MY3, MOST104 – 2320-B-038 – 020-MY3) and Academia Sinica for technical assistance.

Keywords: Phalaenopsis, LC-MS, SIMCA-P+, Metabolomics, light qualities.

References:

[1] Janick J, WhipkeyA. Trends in new crops and new uses. 1st edition. Virginia: ASHS Press; 2002: 458 – 465

[2] Alabadí D, Blázquez MA. Molecular Interactions between light and hormone signaling to control plant growth. Plant Mol Biol 2009; 69: 409 – 17