Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596201
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Isolation and identification of a new dammarane-type triterpene saponin from Panax ginseng

DG Lee
1   Department of Integrative Plant Science, Chung-Ang University, 17546 Anseong, Republic of Korea
,
S Cho
1   Department of Integrative Plant Science, Chung-Ang University, 17546 Anseong, Republic of Korea
,
J Lee
1   Department of Integrative Plant Science, Chung-Ang University, 17546 Anseong, Republic of Korea
,
JP Rodriguez
1   Department of Integrative Plant Science, Chung-Ang University, 17546 Anseong, Republic of Korea
,
IH Cho
2   Department of Convergence Medical Science, College of Oriental Medicine, Kyung Hee University, 02447 Seoul, Republic of Korea
,
HJ Kim
3   Department of Clinical Pharmacology, Soonchunhyang University, 31538 Cheonan, Republic of Korea
,
SW Lee
3   Department of Clinical Pharmacology, Soonchunhyang University, 31538 Cheonan, Republic of Korea
,
YO Kim
3   Department of Clinical Pharmacology, Soonchunhyang University, 31538 Cheonan, Republic of Korea
,
CG Park
3   Department of Clinical Pharmacology, Soonchunhyang University, 31538 Cheonan, Republic of Korea
,
S Lee
1   Department of Integrative Plant Science, Chung-Ang University, 17546 Anseong, Republic of Korea
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 

Panax ginseng (Araliaceae) is a traditional medicinal plant that has been used therapeutically for thousands of years in East Asia. The name “Panax” means “all healing” or “all cure” which describes the traditional belief that ginseng can heal all aspects of the body [1]. The root of P. ginseng, a Korean traditional medicine, is used in both raw and processed forms due to their different pharmacological activities [2]. As part of a continued chemical investigation of ginseng, the focus of this research is on the isolation and identification of compounds from P. ginseng root by open column chromatography, medium pressure liquid chromatography (MPLC), and semi-preparative-high performance liquid chromatography. A phytochemical constituent was isolated from root of P. ginseng by column chromatography and MPLC. This compound was identified by 1H-, 13C- NMR, HMBC, and negative LC-MS, and comparison with other literature[3,4]. Compound 1 was elucidated as a member of the dammarane-type triterpene saponins. Compound 1 from P. ginseng was isolated for the first time. Compound 1 was obtained as a white amorphous powder. Its molecular formula was determined to be CnHnOn based on the FAB-MS and NMR. The 1H-NMR spectrum showed one olefinic, four anomeric, and a glycosyl moiety signal. In the 13C-NMR spectrum, the chemical shifts of four anomeric carbons were observed. Acid hydrolysis of 1 yielded two glycosides. The anomeric carbon signals indicated two glycosyl moieties. As a result, the structure of 1 was elucidated as a new dammarane-type triterpene saponin by spectral data analysis.

Zoom Image
Fig. 1: Structure of new dammarane type skeleton (R1= glycosyl moieties)

Acknowledgements: This work was supported by grants from Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ011582052016), Rural Development Administration, Republic of Korea.

Keywords: Panax ginseng, dammarane ginsenoside, triterpene.

References:

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