Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596195
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Metabolomic studies of Piperaceae species: a correlation between miniaturization and direct extraction strategies

AA Souza Júnior
1   Department of Organic Chemistry, Universidade Estadual Paulista – UNESP, Rua Professor Francisco Degni, 55, 14800 – 900, Araraquara, SP, Brazil
,
LL Zanin
1   Department of Organic Chemistry, Universidade Estadual Paulista – UNESP, Rua Professor Francisco Degni, 55, 14800 – 900, Araraquara, SP, Brazil
,
I Castro-Gamboa
1   Department of Organic Chemistry, Universidade Estadual Paulista – UNESP, Rua Professor Francisco Degni, 55, 14800 – 900, Araraquara, SP, Brazil
,
M Furlan
1   Department of Organic Chemistry, Universidade Estadual Paulista – UNESP, Rua Professor Francisco Degni, 55, 14800 – 900, Araraquara, SP, Brazil
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 

Piperaceae is characterized as a diverse pantropical plant that shows accumulation of biologically active natural products, including amides, lignans, neolignans, benzopyranes, pyrones, flavonoids and terpenoids [1]. Our research group has described in vitro trypanocidal activity of phenolic derivatives from Peperomia obtusifolia [2]. Other important class of biosynthesized compounds from Piper species is amides such as isobutyl, piperidine and piperidone [3]. For example, piplartine, a natural amide, have drawn a lot of interest due to selectively inducing cell death in cancer cells, i.e., do not reduce cell viability in normal cells [4]. Therefore, the metabolites from Piperaceae family exhibit an important function to innovative designs of new pharmaceuticals. This work focused on miniaturization trends in the field of analytical methods for metabolomic approaches. By literature, one of problems that is encountered when develop a metabolomic study is the design of the extraction methodology, including target and untargeted experiments [5]. In such context, our proposal applied a modified method based on miniaturization, direct extraction and pre-treatment samples from species of Piper aduncum, P. tuberculatum, P. arboreum and Peperomia obtusifolia. The results were further evaluated in the duplex context by quantitatively analysis: the yield of extracts and the number of peak detected by HPLC analysis, as indicated in Figure 1, producing greater yields than the conventional methods for the four studied species. The miniaturization and direct extraction approach allowed profiling of a broad array of metabolites, ranging from medium polarity compounds. This improvement in the extraction efficiency can be explained by analytes solvation model. Therefore, this approach provided an efficient and fast methodology for extraction of metabolites, as well as an important contribution towards green chemistry.

Zoom Image
Fig. 1: Graphical representation of the miniaturized extraction method

Acknowledgements: FAPESP, CAPES, and CNPq for financial support. A. A. Souza Jr. thanks FAPESP for a doctoral scholarship (2014/22239 – 8). The authors also thank the financial support provided by FAPESP within the CIBFar/FAPESP. M.F. and I.C.G. thanks CNPq for their own research fellowship.

Keywords: Metabolomic, miniaturization, solid-phase extraction, amides, green chemistry.

References:

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