Applications in natural product chemical profiling and identification using HPLC-NMR and HPLC-MS methodologies

One of the principle goals of the Marine and Terrestrial Natural Product (MATNAP) research group in the School of Science at RMIT University in Melbourne, Australia is the pursuit of new bioactive natural products derived from both marine and terrestrial organisms.

The increasing need to be able to study the crude extracts of these organisms in an efficient manner has directed our attention to the use of rapid chemical profiling strategies. To achieve this we have adopted the use of hyphenated spectroscopic techniques such as HPLC-NMR and HPLC-MS to conduct phytochemical profiling of a range of Australian endemic plants as well as dereplication studies of a number of southern Australian marine algae [1,2]. This presentation will provide an overview of some of these chemical profiling studies. Also a number of unstable natural products identified by these methodolgies, which would otherwise never have been possible, will also be highlighted.

Acknowledgements: Support and sponsorship awarded by the School of Science, RMIT University and the University of Copenhagen is gratefully acknowledged.

Keywords: HPLC-NMR, HPLC-MS, natural products, chemical profiling, dereplication.

References:

[1] Brkljača R, Göker E, Urban S. Dereplication and chemotaxonomical studies of marine algae of the Ochrophyta and Rhodophyta phyla. Mar Drugs 2015; 13: 2714 – 2731

[2] Brkljača R, Urban S. HPLC-NMR and HPLC-MS profiling and bioassay-guided identification of secondary metabolites from the Australian plant Haemodorum spicatum. J Nat Prod 2015; 78: 1486 – 1494