Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596134
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Metabolite profiling and targeted isolation of markers for a comprehensive standardisation of a multi-herb TCM formula to treat atopic dermatitis

J Houriet
1   School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Quai Ernest-Ansermet 30, 1211 Geneva 4, Switzerland
,
E Ferreira Queiroz
1   School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Quai Ernest-Ansermet 30, 1211 Geneva 4, Switzerland
,
PM Allard
1   School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Quai Ernest-Ansermet 30, 1211 Geneva 4, Switzerland
,
L Vallin
1   School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Quai Ernest-Ansermet 30, 1211 Geneva 4, Switzerland
,
S Li
2   Medical Corporation Soujikai, Chuo-ku, Hirano 2 – 2-2, 541 – 0046 Osaka, Japan
,
R Wang
3   Zhejiang CONBA Pharmaceutical & Drug Research Development Corporation, Hangzhou 310052, Zhejiang, China
,
L Marcourt
1   School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Quai Ernest-Ansermet 30, 1211 Geneva 4, Switzerland
,
K Kuchta
4   National Institute of Health Sciences, Division of Pharmacognosy, Phytochemistry and Narcotics, Setagaya-ku, Kamiyoga 1 – 18 – 1, 158 – 8501 Tokyo, Japan
,
JL Wolfender
1   School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Quai Ernest-Ansermet 30, 1211 Geneva 4, Switzerland
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

 

Atopic dermatitis (atopic eczema) is a common inflammatory skin disorder, treated in Western medicine by topical corticosteroids and emollients [1]. Recently, an open label clinical study showed the efficacy of a new formula based on a mixture of ten herbs used in Traditional Chinese Medicine (TCM) [2]. The quality control for such a multi-herb mixture is challenging. The aim of this work was to design an innovative strategy for a comprehensive and rational standardisation. It is based on an in depth high resolution metabolite profiling of the TCM formula, localisation of chemical markers by data mining and targeted preparative isolation of standards directly from the complex TCM mixture. The TCM formula and all single plant extracts were analysed by UHPLC-PDA-ELSD-HRMS. The UHPLC-HRMS metabolic fingerprints allow the detection of more than one thousand m/z features. The comparison of the single extracts and TCM, following peak picking of all these features, with in depth annotation provided all the necessary data for the selection of specific makers for standardisation. ELSD detection was used as a filter for a selection of the more abundant markers of each plant for targeted isolation. For a rational and rapid isolation, the TCM formula was fractionated after an enrichment procedure at large scale by MPLC. For this, the analytical HPLC-PDA-ELSD conditions were transferred geometrically to the preparative MPLC-UV-ELSD using chromatographic calculations, ensuring the same selectivity at both scales [3]. At-line high throughput UHPLC fingerprint of all fractions merged in a MPLCxUHPLC plot enabled the localisation and further purification of the target markers. This provided all the necessary information for the development of the quality control method that could thus efficiently take into consideration the complexity of the multi-herb preparation and give a rational for standardisation.

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Keywords: UHPLC-PDA-ELSD-HRMS, multi-herb quality control, standardisation, Traditional Chinese Medicine, dereplication, metabolomics.

References:

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