J Neurol Surg B Skull Base 2016; 77 - FP-11-02
DOI: 10.1055/s-0036-1592491

Proteomic Profiling in Chordoma

Youssef Yakkioui 1, Jacobus. J. van Overbeeke 1, Yasin Temel 1
  • 1Department of Neurological Surgery, Maastricht University Medical Center, Maastricht, The Netherlands

Objective: Chordoma are a malignant group of neoplasms that are believed to arise from remnants of the embryonic notochord. Disease management in individuals suffering from this disease is challenging as multimodal therapy often remains insufficient to prevent tumor regrowth. Therefore, researchers are focused on the development of novel biomarkers for targeted therapy. Most of the research in this perspective is performed on the level of the genome and transcriptome. This study focuses on the proteome of chordomas.

Methods: Using Isobaric tags for relative and absolute quantitation (iTRAQ) we have identified the proteomic profile in chordomas (n = 4) compared with chondrosarcomas (n = 4). In addition, the chordoma proteome (n = 4) was compared with that of embryonic intervertebral disk tissue (N = 4).

Results: Our experiments show that KRT18, KRT19 and S100A10 proteins are particularly enriched in the whole proteome analysis when chordomas are compared with both control tissues, with average volt changes of 2.9, 5, and 3.4. Proteomic profiling in addition revealed proteins involved in cell matrix organization, protein folding and cell signaling.

Conclusion: Taken together, this study provides confirmation of iTRAQ methodology in detecting novel targets and offers an integrative tumor total/glycomic/phosphoproteomic profiling for chordoma. In addition, features revealed by our work yield novel insights in chordoma genesis and provide potential candidates for the use targeted therapy.