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Int J Angiol 2017; 26(01): 049-052
DOI: 10.1055/s-0036-1588062
Original Article
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Analysis of Apolipoprotein B Protein of Circulating Multiple-Modified Low-Density Lipoprotein

Emile R. Zakiev
1   Laboratory of Angiopathology, Institute of General Pathology and Pathophysiology, Moscow, Russia
,
Vasily N. Sukhorukov
1   Laboratory of Angiopathology, Institute of General Pathology and Pathophysiology, Moscow, Russia
,
Ekaterina A. Ivanova
2   Department of Development and Regeneration, KU Leuven, Leuven, Belgium
,
Alexander N. Orekhov
1   Laboratory of Angiopathology, Institute of General Pathology and Pathophysiology, Moscow, Russia
3   Institute for Atherosclerosis Research, Skolkovo Innovation Center, Moscow, Russia
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Publikationsdatum:
26. Dezember 2016 (online)

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Abstract

Modified low-density lipoprotein (LDL) is the main source of lipid accumulation in the arterial wall affected by atherosclerosis. We aimed to compare the properties of apolipoprotein B (apoB) from native and modified LDL. Modified (desialylated) LDL and native LDL were extracted from blood of atherosclerotic patients. We characterized apoB structure of LDL particles in total LDL preparation, circulating modified LDL (cmLDL), and native LDL. Intact cmLDL had a twofold lower content of free amino groups than native LDL. Delipidated apoB from cmLDL also had a lower content of free amino groups. The rates of tryptic hydrolysis and elastase digestion of cmLDL were twofold higher in comparison to native LDL. Therefore, cmLDL from atherosclerotic patients had altered apoB properties. Our observations strengthen the hypothesis of multiple modification of LDL in the bloodstream and underscore the importance of desialylated LDL as a possible marker of atherosclerosis.

Keywords

atherosclerosis - low-density lipoprotein - modified LDL - apolipoprotein B - desialylation
 

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