Development and Evaluation of a Computer-Controlled System to Assess Associative Visual Learning and Memory in Zebrafish Larvae

Background: Understanding the underpinnings of learning and memory both on the molecular and circuit level is facilitated by standardized behavioral assays preceding both pharmacological and genetic tests. Here, we characterize the process of designing, development and evaluation of a new method for computer based analysis of associative learning in Zebrafish of all age groups. The experiment is also the first example of associative learning in Zebrafish larvae which were only object of non-associative learning and memory assays up to date. The present data suggest that Zebrafish of all age groups are able to associate a visual stimulus with negative reinforcement and recall the memories after hours and days. Moreover, the data show experimental extinction of memories.

Methods: The controls experiencing unpaired presentation of stimulus and reinforcement were not able to make this association. Thus, we suggest that the present computerized experiment is much likely suitable as a high-throughput genetic or pharmacological screening test.