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Int Arch Otorhinolaryngol 2016; 20(04): 370-376
DOI: 10.1055/s-0036-1579742
Original Research
Thieme Publicações Ltda Rio de Janeiro, Brazil

Curcumin Reduces the Noise-Exposed Cochlear Fibroblasts Apoptosis

Tengku Siti Hajar Haryuna
1   Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan 20155, Indonesia
,
Wibi Riawan
2   Department of Biochemistry, Faculty of Medicine, Universitas Brawijaya, Malang 65145, Indonesia
,
Ardyansyah Nasution
1   Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan 20155, Indonesia
,
Suprapto Ma'at
3   Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya 60131, Indonesia
,
Juliandi Harahap
4   Department of Community Medicine, Faculty of Medicine, Universitas Sumatera Utara, Medan 20155, Indonesia
,
Indri Adriztina
1   Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan 20155, Indonesia
› Author Affiliations
Further Information

Publication History

03 October 2015

11 December 2015

Publication Date:
04 March 2016 (online)

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Abstract

Introduction The structural changes underlying permanent noise-induced hearing loss (NIHL) include loss of the sensory hair cells, damage to their stereocilia, and supporting tissues within the cochlear lateral wall.

Objective The objective of this study is to demonstrate curcumin as a safe and effective therapeutic agent in the prevention and treatment for fibroblasts damage within the cochlear supporting tissues and lateral wall through cell death pathway.

Methods We divided 24 Rattus norvegicus into 4 groups, Group 1: control; Group 2: noise (+); Group 3: noise (+), 50 mg/day curcumin (+); Group 4: noise (+), 100 mg/day curcumin (+). We provided the noise exposure dose at 100 dB SPL for two hours over two weeks and administered the curcumin orally over two weeks. We examined all samples for the expressions of calcineurin, nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), and apoptotic index of cochlear fibroblasts.

Results We found significant differences for the expressions of calcineurin (p < 0.05) in all groups, significant differences for the expressions of NFATc1 (p < 0.05) in all groups, except in Groups 1 and 4, and significant differences for the apoptotic index (p < 0.05) in all groups.

Conclusion Curcumin proved to be potentially effective in the prevention and treatment for fibroblasts damage within the cochlear supporting tissues and lateral wall regarding the decreased expression of calcineurin, NFATc1, and apoptotic index of cochlear fibroblasts.

Keywords

noise - curcumin - calcineurin - NFATc1 - apoptosis - cochlea
 

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