Planta Med 2016; 82 - PC59
DOI: 10.1055/s-0036-1578761

Avicularin As The Major Hypoglycemic Compound Isolated From Guava Leaves

W Ouyang 1, 3, XA Zhu 2, XH Shao 2, Y Cao 2
  • 1School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, P. R. China
  • 2College of Food Science, South China Agricultural University, Guangzhou 510642, P. R. China
  • 3National Center for Natural Products Research, University of Mississippi, MS 38677, USA.

In order to investigate the hypoglycemic constituents of guava leaves, a vitro glucose-uptake promoting ability bioassay model [1] was used to guide the column chromatography and the chemical structures were identified based on their spectroscopic data. The results indicated that the ethyl-acetate extract showed the best activity and was further separated into 11 fractions; Five pure compounds were isolated from the most active fractions Fr. 4 and determined to contain hyperoside, isoquercitrin, quercetin-3-O-β-D-xylpyranoside, guaijaverin and avicularin, respectively; avicularin showed the highest hypoglycemic effect in a dose-dependent manner with the EC50 at 14.27µM. it was concluded that avicularin as the major hypoglycemic compound in guava leaves may play an important role on anti-diabetic ability of guava leaves extract.

Acknowledgements: This research was financially supported by the National Natural Science Foundation of China (31201321) and the Education Department of Hunan Province (B13084). Support for Wen Ouyang from the China Scholarship Council is gratefully acknowledged.

References: [1] Adachi Y, Sakurai H, et al. (2004) Chem. Pharm. Bull., 52: 428 – 433.