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Apoptotic Effects Of Rhus Punjabensis Stewart On HL-60 Leukemia Cells
Many of today's cancer drugs are from natural origin; thus, plant extracts represent great potential as a source of new molecules with potential therapeutic application . Rhus punjabensis Stewart (Anacardiaceae) is a small tree belonging to the genus Rhus  and can be found in temperate and tropical regions worldwide . The leaves of this plant were collected in Northern Pakistan where it is used as a medicinal plant in folk medicine to treat hepatitis and other ailments. In the screening efforts for new natural compounds with anticancer properties, we report here a study that aims to evaluate the mechanism of action for compound 4, isolated from the leaves of R. punjabensis. Compound 4 exhibited significant cytotoxic effects against HL-60 human myeloid leukemia cells with an ED50 value of 1.1 µg/mL. Thus, the signaling cellular effects of compound 4 on HL-60 cells will be studied by using whole cell based assays, flow cytometry, and western blot for the analysis of reactive oxygen species (ROS), nuclear factor kappa B (NF-κB p65 and p50), mitochondrial transmembrane potential (MTP), and cell cycle. Compound 4 was identified as a significantly bioactive triterpene secondary metabolite from this plant with great potential for cancer drug development.
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