Effect Of Electro-Acupuncture Pretreatment At Neiguan Point On NO, NOS Of Rabbit Serum During Myocardial Ischemia-Reperfusion

Objective: To observe the effect of electro-acupuncture pretreatment at Neiguan Point (PC6) on the Nitric Oxide (NO), Nitric Oxide Synthase (NOS) of rabbit serum (which are endogenous protective substances) during experimental myocardial ischemia-reperfusion injury. Methods: To divide 40 New Zealand white rabbits (half male and half female) randomly into sham group, ischemia-reperfusion model group, Electro-acupuncture Pretreatment at Neiguan Point group, Electro-acupuncture pretreatment at Lieque Point group, Electro-acupuncture Pretreatment at Hegu Point group. Create a model of myocardial ischemia-reperfusion by coronary artery ligation, 30 min of ligation and 60 min of reperfusion; each of the Electro-acupuncture Pretreatment groups have been pretreated by electro-acupuncture for 5 days (20 min/day) before coronary artery ligation. Using Enzyme-Linked Immunosorbent Assay (ELISA) to detect the levels of NO, NOS in the serums of each group. Results: compared with the sham group, the levels of NO, NOS in the serums of the ischemia-reperfusion group decrease (P < 0.05); compared with the ischemia-reperfusion group, the levels of NO, NOS in the serums of the Electro-acupuncture Pretreated at Neiguan Point group increase significantly (P < 0.01). Conclusion: Electro-acupuncture Pretreatment at Neiguan Point, Pericadium Meridian can effectively raise the levels of myocardial NO, NOS [1].

Acknowledgements: Thanks to Professor Jie Yan for experimental design and graduate students Zhao Liu for carrying out the experiments and data analysis. Support for this research from the National Natural Science Foundation of China (No. 81102661 and No. 81072868) is gratefully acknowledged.

References: [1] Wang W, Li J et al. (2015)J Tradit Chin Med, 35(5):577 – 582.