Analysis For Valuable Compounds And Specific Proteins From Korean Herbal Medicine As Known Siberian Ginseng (Eleutherococus Sp.)

Siberian ginseng (Eleutherococcus Sp.) is officially included in the Korean pharmacopoeia (KP) and is used as stem bark or root bark for medicinal purpose [1]. In Korean medicine, it was collected and dried with sunlight between summer and fall in proper ways. Korean herbal medicine known as Siberian ginseng (Eleutherococcus Sp.) was analyzed for valuable proteins by using TLC, HPLC, and SDS-PAGE. To analyze the specific protein extraction, it was performed using MALDI-TOF-MS/MS. This experiment's target is analogizing useful compounds and protein elements which are included in the several Eleutherococus sp. And the main ingredients were the standard compounds: Eleutheroside, Sesamin, and Chiisanoside. First, the samples were analyzed by TLC and HPLC after sampling from fields. Each extraction liquids of leaf, stem, and root from Eleutherococus sp. were differentially contributed. Chiisanoside was included in leaf of all three Eleutherococus sp. and Sesamin was involved in stem/root of the Eleutherococus sp. However, Eleutheroside (E) was not detected in leaf of all three species. The gels of 2DE-PAGE from all species were varied and the spots of interests in specific proteins were analyzed and performed by MALDI-TOF-MS/MS. This study was limited to make proper identification because of less protein database. To future study, protein database of Eleutherococus sp. should be developed for acquired identification of protein spots.

Acknowledgements: This work was supported by Establishment of Molecular Authentication System and Development of Seedling Proliferation Techniques for the Production of Authentic Herbal Medicines (K15421), the Korea Institute of Oriental Medicine (KIOM) to the Ministry of Science, ICT & Future Planning (MSIP). Also, it was supported by Bioscience team of Research & Education (R & E), Daejeon Dongsin Science High school, Daejeon Metropolitan Office of Education, Republic of Korea.

Reference: [1] The Korean Pharmacopoeia 10th edition. The KFDA Notification No. 2012 – 129. 2012. Dec 27th. Korea Food & Drug Administration.