Planta Med 2016; 82 - PA16
DOI: 10.1055/s-0036-1578631

Identification And Quantification Of Chemical Constituents From Indian Bee Propolis Extracts Using HPTLC And UHPLC-DAD-MS

S Sagi 1, B Avula 1, MH Masoodi 2, A Farooq Wali 2, IA Khan 1, 3
  • 1National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, University, MS 38677, USA
  • 2Department of Pharmaceutical Sciences, Faculty of Applied Sciences & Technology, University of Kashmir, Srinagar – 190006, J & K, India
  • 3Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS 38677, USA

Bee propolis is a resinous, gummy and balsamic material collected from flora by honey bees (Apis mellifera L. belongs to Apidae family). Bee propolis is official in the United States Pharmacopeia and natural health product monograph Canada (USP. 2009), (Health Canada 2009). The main phytoconstituents reported to have been isolated from the bee propolis are polyphenolics, chalcone, triterpenes, coumarins, aromatic acids and their esters [1, 2]. The reference standards caffeic acid (1), trans-ferulic acid (2), p-coumaric acid (3), quercetin (4), luteolin (5), Naringenin (6), apigenin (7), kaempferol (8), Pinocembrin (9), caffeic acid phenethyl ester [CAPE] (10), chyrsin (11), pinobanksin-3-O-acetate (12) acacetin (13) and galangin (14) were used to develop an HPTLC chemical fingerprint profile and UHPLC-DAD-MS method for quantification of chemical constituents (1 – 14) from Indian Bee Propolis extracts and dietary supplements. Seven bee propolis extracts and 14 dietary supplements claiming to contain bee propolis extracts were analyzed. Content of chemical constituents (1 – 14) varied from 0.01 – 9.58 mg/100 mg extract and 0 – 14.06 mg/day for bee propolis extracts and dietary supplements respectively. The results indicated that commercial products are of variable quality.

Acknowledgements: This research is supported in part by “Science Based Authentication of Dietary Supplements” funded by the Food and Drug Administration grant number 1U01FD004246-05, the United States Department of Agriculture, Agricultural Research Service, Specific Cooperative Agreement No. 58-6408-1-603-07.

References: [1] Huang S, Zhang C-P, et al. (2014) Molecules, 19: 19610 – 19632. [2] De Groot AC, Popova MP, et al. (2014) Wapserveen, The Netherlands: acdegroot publishing, 2014, 11 pages. ISBN/EAN: 97-90-813233-0-7.