Planta Med 2015; 81 - PW_236
DOI: 10.1055/s-0035-1565860

Comprehensive evaluation of the quality of Platycodi Radix with reference standard extract

L Zeng 1, H Kong 1, M Zhu 2, W Yan 1
  • 1Department of Chemistry, Zhejiang University, Hangzhou, China
  • 2Zhejiang Institute for Food and Drug Control, Hangzhou, China

Reference standard extract (RSE), a new type of the reference substance, typically consisted of main components from raw material [1]. Platycodi Radix mainly contains platycosides [2]. In this study, a new quality control method employed RSE to comprehensively evaluate quality of herbal medicine was proposed. Through preparation and standardization of the platycosides RSE in which the content of total platycosides was more than 95%, the quality of Platycodi Radix was evaluated as follows:

Section 1: Identification. Five main platycosides from Platycodi Radix were clearly identified by TLC with R fs of 0.21, 0.30, 0.40, 0.52 and 0.65. Nine platycosides were identified by characteristic HPLC chromatogram with relative retention time (RRT) as 0.32, 0.36, 0.55, 0.91, 1.00, 1.10, 1.17, 1.40 and 1.48 (Fig. 1).

Section 2: Quantitative determination of the platycosides with HPLC-ELSD. Contents of nine platycosides in Platycodi Radix were analyzed using standardized RSE obtained in this work. The results showed that contents of the nine platycosides were ranging from 0.02 to 0.7%, which was also confirmed by conventional method using nine reference standards.

RSE could provide more comprehensive chemical information to assess the quality of complex characteristics of herbal medicine, compared with conventional quality-assessment methods, and also relive the stress of searching for pure reference standards.

Fig. 1: Representative HPLC-ELSD chromatogram of Platycodi Radix
A: standard compounds; B: reference standard extract; C: sample; 1: deapioplatycoside E: 2: platycoside E; 3: platycodin D3; 4: deapioplatycodin D; 5: platycodin D; 6: polygalacin D; 7: 3″-O-acetylplatycodin D; 8: 2″-O-acetylplatycodin D2; 9: 2″-O-acetylplatycodin D

References:

[1] Li SP, Qiao CF, Chen YW, Zhao J, Cui XM, Zhang QW, Liu XM, Hu DJ. A novel strategy with standardized reference extract qualification and single compound quantitative evaluation for quality control of Panax notoginseng used as a functional food. J Chromatogr A 2013; 1313: 302 – 307

[2] Ishii H, Tori K, Tozyo T, Yoshimura Y. Saponins from roots of Platycodon grandiflorum. Part 2. Isolation and structure of new triterpene glycosides. J Chem Soc Perkin Trans I 1984; 1384: 661 – 668