Planta Med 2015; 81 - PW_142
DOI: 10.1055/s-0035-1565766

Optimization of bioactive phenolic compounds extraction by modified supercritical fluid

L Gourguillon 1, 2, E Lesellier 2, A Lobstein 1
  • 1Laboratory of Pharmacognosy and Bioactive natural products, UMR CNRS 7200, university of Strasbourg, BP 60024, 67401, Illkirch, France, Strasbourg, France
  • 2BiotechMarine, ZI BP 72, 22260 Pontrieux, France
  • 3Analytical and organic chemistry institute ICOA, UMR 7311, UMR 7311 University of Orléans, BP 6759, rue de Chartres, 45067 Orléans, France

Extraction by supercritical fluid is an environmentally friendly method by comparison with the conventional liquid/liquid or solid/liquid extraction. Moreover the physicochemical properties of supercritical CO2 (viscosity, diffusivity and surface tension) [1], with addition of polar co-solvents, favors the extraction of phenolic compounds [2, 3].

Supercritical fluid extraction of bioactive phenolic compounds such as caffeic acids derivatives identified by dereplication with LC-DAD-MS2 in a halophytic Asteracae, was tested. 100% CO2 and CO2 mixed with co-solvent were evaluated. Two temperatures were tested and a kinetic study of the extraction was made. Total extraction yields were measured and the major dicaffeoylquinic acids content was analyzed by HPLC-DAD-DEDL.

Presence of 10% ethanol in CO2 is not enough to extract polar compounds of interest; the addition of 20% water in ethanol provides a strong enhancement of the extraction of dicaffeoylquinic acids. Moreover, in these conditions, it is not necessary to increase temperature to extract phenolic compounds. Kinetic study shows that in 1h, 80% of final extract mass is obtained.

The results show that supercritical modified fluids (CO2/EtOH/water) are very interesting for finding an alternative to the organic solvents used in the fields of cosmetics and food.

Fig. 1: RP-HPLC chromatographic profiles of halophytic Asteraceae extracts obtained by SFE with different conditions (198nm)
a-100% C02
b- 90% C02 + 10% EtOH
c- 90% C02 + 10% EtOH/H2O (80/20) (v:v)

References:

[1] Azmir J, Zaidul ISM, Rahman MM, Sharif KM, Mohamed A, Sahena F, Jahurul MHA, Ghafoor K, Norulaini NAN, Omar AKM. Techniques for extraction of bioactive compounds from plant materials: A review. J Food Eng 2013; 117: 426 – 436

[2] Diaz-Reinoso B, Moure A, Dominguez H, Parajo JC. Supercritical CO2 extraction and purification of compounds with antioxidant activity. J Agric Chem 2006; 54: 2441 – 2469

[3] Heleno SA, Martins A, Queiroz MJRP, Ferreira ICFR. Bioactivity of phenolic acids: Metabolites versus parent compounds: A review. Food Chem 2015; 173: 501 – 513