Planta Med 2015; 81 - PW_104
DOI: 10.1055/s-0035-1565728

Two new flavone glycosides from the aerial parts of Platycodon grandiflorum and their cytotoxic activity against human tumor cell lines

EB Kim 1, IS Kim 1, HS Kim 1, JH Kwak 1
  • 1School of Pharmacy, Sungkyunkwan University, Suwon, Korea, Republic of (South)

Platycodon gradiflorum A. DC., which belongs to the family Campanulaceae, is a perennial herb and distributed in Korea, China and Japan [1]. The root of P. grandiflorum, a well-known traditional Chinese medicine, has been used for treatment of cough, bronchitis, sore throat and purulent disease [2]. It is widely cultivated in Korea, and has been used more often for food than for medicinal purposes since old times [2]. In the course of a search for bioactive compounds from natural products, we have found that the EtOH extract of P. grandiflorum has cytotoxic activity against human tumor cell lines (MCF-7, SK-OV-3 and Ishikawa) with IC50 below 73 µg/mL. Two new flavone glycosides (1, 2) and six known flavonoids (3-8) were isolated from the EtOAc and n-BuOH fractions of the EtOH extract. The structures of two new compounds, named dorajiside A and B, were determined as luteolin 7-O-(6"-O-acetyl-ß-D-glycopyranoside), 3'-O-α-L-rhamnopyranoside (1) and apigenin 7-O-(6"-O-acetyl-ß-D-glucopyranoside), 4'-O-α-L-rhamnopyranoside (2) from spectral data and chemical evidence. The known compounds, luteolin 7-O-ß-D-glucopyranoside, 3'-O-α-L-rhamnopyranoside (3), apigenin 7-O-ß-D-glycopyranoside, 4'-O-α-L-rhamnopyranoside (4), luteolin 7-O-(6"-O-acetyl)-ß-D-glucopyranoside (5), luteolin 7-O-ß-D-glucopyranoside (6), apigenin 7-O-ß-D-glucopyranoside (7), and luteolin (8) were identified by comparing their spectral data with literature values. Cytotoxicity of isolated compounds were evaluated by using MTT assay against human tumor cell lines (MCF-7, SK-OV-3 and Ishikawa), and most of compounds revealed moderate activity at concentrations below 50 µg/mL.

References:

[1] Lee WT. Colored standard illustrations of Korean plants. Seoul: Academy Book; 1996: 342

[2] Jeong C-H, Choi GN, Kim JH, Kwak JH, Kim DO, Kim YJ, Heo HJ. Antioxidant activities from the aerial parts of Platycodon grandiflorum. Food Chem 2010; 128: 278 – 282