Susceptibility of animal pathogenic bacteria to an ethanolic extract from jambolan (Syzygium cumini) leaves

Pyoderma is a prevalent disease in dogs and its treatment usually involves antimicrobial therapy. Due to the development of resistant bacterial strains, there is an increased interest to study alternative antibacterial compounds. S. cumini L. Skeels is popularly used to treat infectious diseases. This work was aimed at testing the antimicrobial properties of an ethanolic extract from jambolan leaves against Staphylococcus intermedius and Pseudomonas spp. isolated from canine skin infections showing antibiotic resistance. Leaves of S. cumini were collected in Lagoa Bonita Farm, Federal District, Brazil. Air-dried leaves were powdered and exhaustively extracted with ethanol at room temperature for 5 days. Extracts were filtered and the solvent removed by evaporating at 50 °C. The antimicrobial activity the extract was evaluated in 96-well micro dilution plates, and MICs of 0,3 µg/µL and 0,08 µg/µL were found for Pseudomonas spp. and S. intermedius, respectively. Hence, S. cumini should be further investigated with respect to its potential against important veterinary bacterial strains.