Planta Med 2015; 81 - PM_201
DOI: 10.1055/s-0035-1565578

In vitro anticariogenic effects of Rubus caesius extracts and their quality evaluation by HPLC-DAD-MS3 analysis

DM Grochowski 1, A Wiater 2, M Pleszczyńska 2, M Tomczykowa 3, S Granica 4, M Tomczyk 5
  • 1Students' Scientific Association, Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Bialystok, ul. Mickiewicza 2a, 15 – 230, Bialystok, Poland
  • 2Department of Industrial Microbiology, Institute of Microbiology and Biotechnology, Maria Curie-Sklodowska University, ul. Akademicka 19, 20 – 033, Lublin, Poland
  • 3Department of Organic Chemistry, Faculty of Pharmacy, Medical University of Bialystok, ul. Mickiewicza 2a, 15 – 222, Bialystok, Poland
  • 4Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy, Warsaw Medical University, ul. Banacha 1, 02 – 097, Warsaw, Poland
  • 5Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Bialystok, ul. Mickiewicza 2a, 15 – 230, Bialystok, Poland

The genus Rubus (Rosaceae) comprises around 700 species, naturally occurring in temperate climate. Rubus species have been used in traditional medicine for their many medicinal properties [1, 2]. In this study, for the first time, we investigated in vitro inhibitory effects of R. caesius extracts and their sub-fractions against mutans Streptococci. The diethyl ether sub-fraction showed bacteriostatic and bactericidal activities, with minimum inhibitory concentration (MIC) of 1 mg/mL and minimum bactericidal concentration (MBC) in the range of 2 – 4 mg/mL. Furthermore, the diethyl ether fraction inhibited biofilm formation by Streptococci in dose-dependent manner. However, it was also found that all R. caesius preparations exhibited diverse inhibitory effects on de novo synthesis of water-insoluble and water-soluble α-D-glucans by glucosyltransferases of mutans group Streptococci. The examination of phytochemical profile of investigated samples using HPLC-DAD-MS3 revealed that all extracts contained polyphenols: tannins or flavonol derivatives as major constituents. Among identified polyphenolic compounds ellagitaninns, especially sanguniin H-6 or kaempferol and quercetin derivatives have been described as dominant. The results demonstrate that R. caesius extracts could become useful supplements for pharmaceutical products as a new anticariogenic agent in a wide range of oral care products.

References:

[1] Rejewska A, Sikora A, Tomczykowa M, Tomczyk M. Rubus caesius. Phcog Commn 2013; 3: 55 – 57

[2] Paduch R, Tomczyk M, Wiater A, Dudek A, Pleszczyńska M, Tomczykowa M, Granica S, Grochowski DM, Kowalski P. The influence of extracts from Rubus caesius leaves on human colon carcinoma cells cultured in vitro. Planta Med 2014; 80: 1423