Secondary metabolites from Scorzonera latifolia roots

Scorzonera latifolia (Fisch. & Mey.) DC. which belongs to the Asteraceae family, is widely distributed in the Middle and East part of Anatolia as well as Caucasia and Iran [1]. Roots of this plant have been used as analgesic and antihelmintic internally and against women infertility externally in Turkish folk medicine [2]. Analgesic, anti-inflammatory, wound healing and antioxidant activities of S. latifolia have been reported previously [3]. In current study total methanolic extract of the roots were prepared and partitioned by liquid-liquid extraction succesively by petroleum ether, chloroform and ethyl acetate. Ethyl acetate extract was subjected to silicagel column to obtain several subfractions. Further separation procedures on subfractions allowed us to obtain nine phenolic compounds. Structures of the compounds were elucidated by spectroscopic techniques (¹H, ¹³C and 2D-NMR, MS) and identified as chlorogenic acid, chlorogenic acid methyl ester, 1,5-dicaffeoyl quinic acid, 3,5-dicaffeoyl quinic acid, methylester of 3,5-dicaffeoyl quinic acid, hydrangenol-8-O-glucoside, hydrangenol-4'-O-glucoside, scorzotomentosin-4'-O-glucoside and a new isocoumarine derivative named dihydromalicine-4'-O-glucoside.

References:

[1] Baytop T. Türkiye'de Bitkiler ile Tedavi (Theraphy with Medicinal Plants in Turkey). İstanbul: Nobel publishers; 1999: 236 – 237

[2] Chamberlain DF. Scorzonera L. In: Davis PH, editor. Flore of Turkey and The East Aegean Islands. Vol. 5. Edinburgh: University Press; 1975: 632 – 657

[3] Küpeli Akkol E, Bahadır Acıkara Ö, Süntar İ, Saltan Çitoğlu G, Keleş H, Ergene B. Enhancement of wound healing by topical application of Scorzonera species: Determination of the constituents by HPLC with new validated reverse phase method Determination of Phenolic Acids and Flavonoids and Anti-inflammatory Activity of Scorzonera species. J Ethnopharmacol 2011; 137: 1018 – 1027