Planta Med 2015; 81 - PM_122
DOI: 10.1055/s-0035-1565499

Secondary metabolites from Pulsatilla patens and Pulsatilla vulgaris and their biological activity

G Laska 1, A Sienkiewicz 1, M Stocki 2, V Sharma 3, J Zjawiony 3, M Jacob 4
  • 1Department of Environmental Protection and Management, Bialystok University of Technology, Bialystok, Poland
  • 2Department of Forestry in Hajnówka, Bialystok University of Technology, Hajnowka, Poland
  • 3Department of Biomolecular Sciences, School of Pharmacy, University of Mississippi, Oxford, United States
  • 4National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, United States

The aim of this research was the identification of biologically active secondary metabolites isolated from rare plant species Pulsatilla patens (L.) Mill. and cultivated Pulastilla vulgaris Mill. (Ranunculaceae). Chromatographic fractionation of the ethanolic extract of the roots of P. patens resulted in the isolation of two known oleanane-type glycosides identified as hederagenin 3-O-β-D-glucopyranoside and hederagenin 3-O-β-D-galactopyranosyl-(1→2)-β-D-glucopyranoside [1]. The structures of the isolated compounds were determined by 1H and 13C-NMR spectroscopy. Chromatographic analysis using GC-MS of the silylated methanolic extract of the leaves of P. vulgaris revealed the presence of carboxylic acids, such as benzoic, caffeic, malic, and succinic acids [2]. Relative composition (%) of the isolated compounds in total ion current (TIC) was calculated on the basis of peak area in the chromatogram. The extracts of Pulsatilla species were evaluated for their antifungal, antimicrobial, antileishmanial and antimalarial activities. Both P. patens and P. vulgaris were active against the fungus Candida glabrata with IC50 values of 9.37 µg/mL [1] and 11 µg/mL.

References:

[1] Sharma VK, Łaska G, Radhakrishnan SVS, Jacob MR, Zjawiony JK. Phytochemical investigation and pharmacological evaluation of Pulsatilla patens var. patens. Planta Med. 2014; 10: 777

[2] Łaska G, Sienkiewicz A, Stocki M. Secondary metabolites from Pulsatilla species and their role in pharmacology. Planta Med. 2014; 10: 835