Planta Med 2015; 81 - PM_103
DOI: 10.1055/s-0035-1565480

Anti-inflammatory effect of streptochlorin via TRIF-dependent signaling pathways in cellular and mouse models

DW Shim 1, HJ Shin 2, JW Han 1, WY Shin 1, X Sun 1, EJ Sim 1, TJ Kim 3, TB Kang 1, KH Lee 1
  • 1Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju, Korea, Republic of (South)
  • 2Marine Natural Products Chemistry Laboratory, Korea Institute of Ocean Science & Technology, Ansan, Korea, Republic of (South)
  • 3Division of Biological Science and Technology, Yonsei University, Wonju, Korea, Republic of (South)

Streptochlorin, a small compound derived from marine actinomycete, has been shown to have anti-angiogenic, anti-tumor, and anti-allergic activities. However, the anti-inflammatory effects and underlying mechanisms have not yet been reported. In the present study, we investigated the effect of streptochlorin on lipopolysaccharide (LPS)-induced inflammatory responses in vitro and in vivo. As shown in Fig. 1, streptochlorin attenuated the production of proinflammatory mediators such as nitric oxide, cyclooxygenase-2, pro-interleukin (IL)-1β, and IL-6 in LPS-stimulated RAW264.7 cells through inhibition of the Toll/interleukin-1 receptor (TIR)-domain-containing adapter-inducing interferon-β (TRIF)-dependent signaling pathway. IC50 to inhibit those inflammatory mediators is 25 µM.

Fig. 1

To verify the in vivo efficacy of streptochlorin, we injected 0.11˜1.1 mg/kg of streptochlorin by intra peritoneal injection in mouse (Fig. 2). Streptochlorin suppressed the infiltration of immune cells such as neutrophils into the lung and proinflammatory cytokine production such as IL-6 and TNF-α in broncho-alveolar lavage fluid (BALF) in the LPS-induced acute lung injury (ALI) mouse model.

Fig. 2

Taken together, streptochlorin has potent anti-inflammatory effects through regulating TRIF-dependent signaling pathways, suggesting that streptochlorin may provide a valuable therapeutic strategy in treating various inflammatory diseases.