Planta Med 2015; 81 - PM_86
DOI: 10.1055/s-0035-1565463

Alkaloid profiling in Galanthus gracilis from Western Aegean by GC/MS

Ç Karakoyun 1, B Bozkurt 1, Gİ Kaya 1, MA Önür 1, NÜ Somer 1
  • 1Ege University Faculty of Pharmacy Department of Pharmacognosy, Izmir, Turkey

Amaryllidaceae is a family consisting of mostly tropical or subtropical plants with 85 genera and 1100 species. Among the Amaryllidaceae genera growing in Turkey, Galanthus L. possess Amaryllidaceae alkaloids with interesting chemical structures and a range of biological activities. A widespread and amply investigated alkaloid, galanthamine, is a marketed drug for the treatment of Alzheimer's disease. Another widely distributed alkaloid, lycorine has been proven to have prominent biological activities. Galanthus gracilis Celak. is distributed in Western Aegean in Turkey. In the present study, we investigated the chemical constituents of G. gracilis collected from western Aegean (Alankıyı/Bayındır) by using GC/MS. The GC/MS system was operated in the electron impact mode (EI, 70 ev). Helium gas was used as the carrier gas at a constant flow rate 0.8 mL/min. The temperature conditions followed the program: 80 °C for 1 min, 80 – 250 °C at 10 °C/min, 2 min hold at 250 °C, 250 – 300 °C at 10 °C/min and a 10 min hold at 300 °C. İnjector temperature was 250 °C. As a result, 11 alkaloids were detected by GC-MS, including graciline, demethylhomolycorine and tazettine as the major ones.

Acknowledgement: This study was financialy supported by Ege University Research Fund (Project No: 2013/ECZ/018). We thank Ege University, Faculty of Pharmacy, the Research Laboratory of Pharmaceutical Sciences (FABAL) for GC/MS analysis.