Planta Med 2015; 81 - PM_77
DOI: 10.1055/s-0035-1565454

Characterization of antibacterial flavonoids and stilbenes of the root extract of Anogeissus leiocarpus by UHPLC-MS-QTOF

E Salih 1, 2, R Julkunen-Tiitto 3, M Kanninen 2, M Sipi 2, O Luukkanen 2, H Vuorela 1, P Fyhrquist 1
  • 1Faculty of Pharmacy, Division of Pharmaceutical Biosciences, Viikki Biocenter, PO Box 56, FIN-00014 University of Helsinki, Finland, Helsinki, Finland
  • 2Faculty of Agriculture and Forestry, Department of Forest Sciences, Viikki Tropical Resources Institute (VITRI), PO Box 27, FIN-00014 University of Helsinki, Finland, Helsinki, Finland
  • 3Natural Product Research Laboratory, Department of Biology, University of Eastern Finland, 80101 Joensuu, Finland, Helsinki, Finland

Anogeissus leiocarpus occurs in savannas in tropical and subtropical regions of Africa and is used among traditional medicinal practitioners for treatment of various diseases, among them bacterial infections [1]. Eight flavonoids [2], methyl ellagic acid, ellagic acid [4, 5] and the ellagitannins castalagin and flagallonic acid [6] have been reported from the leaves and bark of A. leiocarpus. There are just a few investigations on the phytochemistry of the roots [1], however. This study has aimed on in depth investigations on the phytochemistry of the root part.

Ten microliters of 50 mg/mL methanol extract of the root of A. leiocarpus was applied on RP18 TLC reversed phase. Methanol, water and orthophosphoric acid were used as mobile phase. DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent was used for detecting antioxidative compounds. In order to identify these compounds HPLC-DAD and UHPLC/MS/QTOF methods [3] were used.

Aromadendrin, taxifolin, methyltaxifolin, ampelopsin and eriodictyol as well as pinosylvin and methylpinosylvin and 3,3'-Di-O-methyl ellagic acid glucoside were identified for the first time in the roots of A. leiocarpus. In addition pentagalloyglucose and digalloylglucose were found in the root. We have demonstrated that a methanolic root extract of A. leiocarpus gives promising antibacterial effects. These effects might be connected to antioxidative flavonoids and ellagic acid derivatives in this extract.

Acknowledgements: This study has been supported by Ella and Georg Ehrnrooth Foundation in Finland. This abstract is dedicated to the memory of Professor Raimo Hiltunen (1944 – 2014).


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