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Characterization of antibacterial flavonoids and stilbenes of the root extract of Anogeissus leiocarpus by UHPLC-MS-QTOF
Anogeissus leiocarpus occurs in savannas in tropical and subtropical regions of Africa and is used among traditional medicinal practitioners for treatment of various diseases, among them bacterial infections . Eight flavonoids , methyl ellagic acid, ellagic acid [4, 5] and the ellagitannins castalagin and flagallonic acid  have been reported from the leaves and bark of A. leiocarpus. There are just a few investigations on the phytochemistry of the roots , however. This study has aimed on in depth investigations on the phytochemistry of the root part.
Ten microliters of 50 mg/mL methanol extract of the root of A. leiocarpus was applied on RP18 TLC reversed phase. Methanol, water and orthophosphoric acid were used as mobile phase. DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent was used for detecting antioxidative compounds. In order to identify these compounds HPLC-DAD and UHPLC/MS/QTOF methods  were used.
Aromadendrin, taxifolin, methyltaxifolin, ampelopsin and eriodictyol as well as pinosylvin and methylpinosylvin and 3,3'-Di-O-methyl ellagic acid glucoside were identified for the first time in the roots of A. leiocarpus. In addition pentagalloyglucose and digalloylglucose were found in the root. We have demonstrated that a methanolic root extract of A. leiocarpus gives promising antibacterial effects. These effects might be connected to antioxidative flavonoids and ellagic acid derivatives in this extract.
Acknowledgements: This study has been supported by Ella and Georg Ehrnrooth Foundation in Finland. This abstract is dedicated to the memory of Professor Raimo Hiltunen (1944 – 2014).
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