Planta Med 2015; 81 - PM_36
DOI: 10.1055/s-0035-1565413

Chemical constituents and their bioactivities from the stem of Neolitsea konishii

IS Chen 1, 2, SY Lin 2, TL Cheng 3, HR Liao 4, HS Chang 1, 2, CH Lin 1, SL Wong 1
  • 1School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan
  • 2Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan
  • 3Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan
  • 4Graduate Institute of Natural Products, School of Traditional Chinese Medicine, Chang Gung University, Tao-Yuan, Taiwan

Neolitsea konishii (Hay.) Kanehira & Sasaki (Lauraceae) is a small evergreen tree that is distributed in the Ryukyus and Taiwan. The methanolic extract of the stem of Neolitsea konishii has been shown with anti-eβG (Escherichia coli β glucuronidase) and anti-inflammatory activities. The anti-eβG activity of the stem of N. konishii has not yet been investigated; therefore, the aim of this study is to isolate its chemical constituents and evaluate their biological activities.

Bioassay-guided fractionation of the stem of N. konishii has led to the isolation of one new 1,3-diphenylbutanoid, (-)-konishibutanin and two new lignans, neokoninins A and B, along with 21 known compounds till now. The structures of these isolates were elucidated by spectral methods. Among the isolates, 132-hydroxy-(l32-R)-pheophytin a and N-trans-feruloyl-3',4'-dihydroxyphenylethylamine exhibited potent anti-eβG activity with inhibition ratio of 88% and 76% and with low inhibition of hβG (human β glucuronidase) respectively. In addition, trans-9,9'-O-di-(E)-feruloyl-(-)-secoisolariciresinol showed potent inhibitory U46619 factor and fMLP-induced superoxide generation in human neutrophils activity with IC50 values of 7.89 ± 0.35 and 2.43 ± 0.03 µM.