Planta Med 2015; 81 - PM_33
DOI: 10.1055/s-0035-1565410

In vitro susceptibility of Madurella mycetomatis to the leaves extracts of Terminalia brownii

HA Ali 1, EA Omer 2, SA Khalid 2, 3
  • 1Commission for Biotechnology and Genetic Engineering, National Center for Research, Khartoum, Sudan, Khartoum, Sudan
  • 2Department of Pharmacognosy, Faculty of Pharmacy, University of Khartoum, P.O. Box 1996 Khartoum, Sudan, Khartoum, Sudan
  • 3Faculty of Pharmacy, University of Science and Technology, Omdurman, P.O. Box 1507 Khartoum, Sudan., Khartoum, Sudan

The present communication represents an attempt to investigate the antimycetomal activity of the leaves of Terminalia brownii (Combretaceae) and to define the chemical profiles of the active agents. The antifungal activity of a number of taxa of this family has been attributed to its tannin and saponin contents [1].

Air dried ground leaves of T. brownii were extracted using 80% methanol. The concentrated methanolic extract was sequentially fractionated with petroleum ether, chloroform and ethyl acetate. The aforementioned extract and its respective organic fractions were tested against Madurella mycetomatis employing 96-microplate-based anti-mycetomal assay incorporating resazurin as an indicator [2]. The plant methanolic crude extract showed activity against M. mycetomatis with an MIC of 78.1 µg/mL while the chloroform and ethyl acetate fractions exhibited significant activity with MIC< 39.1 µg/mL.

Reverse phase HPLC coupled with tanden mass spectromentry led to the identification of a tetrahydro chalcone, p-coumaryl glucoside, and catechin 3-O gallate in the ethyl acetate fraction. Additionally, two flavonoids beside the C-glycosylated flavone, isoorientin, and myricetin 3-O-hexose gallate were also identified in this fraction. Combrestastin B5 O-hexose gallate stilbene, in addition to five other prenylated stilbenes were also identifiedt. Ellagic acid was the major compound in the chloroform fraction. Hydroxylated flavan-3-ol gallolcatechin was identified in this fraction along with two polymethoxylated flavonoids.

References:

[1] Baba-Moussa F, Akpagana K, Bouchet P. Antifungal activities of seven West African Combretaceae used in traditional medicine. Journal of Ethnopharmacology 1999; 66: 335 – 338

[2] Khalid SA. Development of microtiter plate-based method for the determination of the MIC of antimycetomal agents against Madurella mycetomatis. II ResNet NPND workshop on natural products against neglected diseases, Nov. 25 – 28th, 2014, Rio de Janeiro, Brazil.