Planta Med 2015; 81 - PM_09
DOI: 10.1055/s-0035-1565386

Meadowsweet (Filipendula ulmaria): LC-MS phenolic characterization and ameliorating effect on cisplatin-induced hepatotoxicity

J Katanić 1, EM Pferschy-Wenzig 2, R Bauer 2, V Mihailović 1
  • 1Department of Chemistry, Faculty of Science, University of Kragujevac, Radoja Domanovica 12, 34000 Kragujevac, Serbia, Kragujevac, Serbia
  • 2Institute of Pharmaceutical Sciences, Department Pharmacognosy, Karl-Franzens University Graz, Universitaetsplatz 4/1, 8010 Graz, Austria, Graz, Austria

The liver plays a major role in detoxifying and clearing xenobiotics that may lead to liver damage and hepatic dysfunction. The present study was carried out to evaluate the effect of the methanolic extracts from aerial parts and roots of Filipendula ulmaria (L.) Maxim. (Rosaceae) (FUR, FUA) on cisplatin-induced hepatotoxicity. Wistar rats were treated for 10 days with FUA and FUR in three doses (100, 200 and 400 mg/kg body weight). Hepatotoxicity was induced on 5th day of treatment with a single injection of cisplatin (7.5 mg/kg, i.p.). Negative and positive control groups were also evaluated. The results show that the treatment with cisplatin (positive control group) increased the level of serum transaminases (ALT, AST). Treatment with both extracts, especially at concentrations of 200 and 400 mg/kg, significantly reduced the enzymatic activity of ALT and AST. Oxidative stress markers in liver tissue like superoxide-dismutase (SOD) and catalase (CAT) activities, as well as levels of reduced glutathione (GSH) and malonyl dialdehyde (MDA), indicated a high level of oxidative damage in the group treated with cisplatin only. The treatment with FUA and FUR significantly (p < 0.05) and dose-dependently alleviated activities of SOD and CAT. Also, GSH level in hepatic tissue was dose-dependently increased due to extracts treatment. Lipid oxidation in livers of the rats treated with F. ulmaria extracts was considerably reduced regarding MDA levels. Based on presented results it can be concluded that the extracts of F. ulmaria possess hepatoprotective activity against CP-hepatotoxicity, which was demonstrated through regulation of oxidative stress markers in serum and hepatic tissue. The phytochemical profile of the extracts was evaluated by LC-DAD-MSn. In FUR catechin as well as procyanidin dimers and trimers and in FUA catechin, epicatechin, hydrolysable tannins (trigalloyl-hexahydroxydiphenol-glucoses), and the flavonoids spiraeoside and quercitrin could be identified.