Planta Med 2016; 82(04): 298-304
DOI: 10.1055/s-0035-1558219
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

In Vitro Activation of eNOS by Mangifera indica (Careless™) and Determination of an Effective Dosage in a Randomized, Double-Blind, Human Pilot Study on Microcirculation

Alexandra Gerstgrasser
1   Vital Solutions Swiss AG, Romanshorn, Schweiz
Sigrid Röchter
1   Vital Solutions Swiss AG, Romanshorn, Schweiz
Dirk Dressler
2   BioTeSys GmbH, Esslingen
Christiane Schön
2   BioTeSys GmbH, Esslingen
Claudia Reule
2   BioTeSys GmbH, Esslingen
Sybille Buchwald-Werner
1   Vital Solutions Swiss AG, Romanshorn, Schweiz
› Author Affiliations
Further Information

Publication History

received 02 September 2015
revised 09 October 2015

accepted 12 October 2015

Publication Date:
19 November 2015 (online)


Mangifera indica fruit preparation (Careless™) activates the evolutionary conserved metabolic sensors sirtuin 1 and adenosine monophosphate-activated protein kinase, which have been identified as playing a key role in microcirculation and endothelial function. Here, an acute effect of a single dose of 100 mg or 300 mg Careless™ on microcirculation was investigated in a randomized, double-blind, crossover pilot study in ten healthy women to determine the effective dosage. Microcirculation and endothelial function were assessed by the Oxygen-to-see system and pulse amplitude tonometry (EndoPAT™), respectively. Cutaneous blood flow was increased over time by 100 mg (54 % over pre-values, p = 0.0157) and 300 mg (35 % over pre-value, p = 0.209) Careless™. The EndoPAT™ reactive hyperemia response was slightly improved 3 h after intake compared to pretesting with 300 mg Careless™. Furthermore, activation of endothelial nitric oxide synthase, as an important regulator for endothelial function, was tested in vitro in primary human umbilical vein endothelial cells. Careless™, after simulation of digestion, increased the activated form of endothelial nitric oxide synthase dose-dependently by 23 % (300 µg/mL), 42 % (1500 µg/mL), and 60 % (3000 µg/mL) compared to the untreated control. In conclusion, the study suggests moderate beneficial effects of Careless™ on microcirculation, which is at least partly mediated by endothelial nitric oxide synthase activation.

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