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Planta Med 2016; 82(04): 356-361
DOI: 10.1055/s-0035-1558211
Natural Product Chemistry and Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

HPLC Method for Chemical Fingerprinting of Guggul (Commiphora wightii) – Quantification of E- and Z-Guggulsterones and Detection of Possible Adulterants

Rida Ahmed
1   National Center for Natural Products Research, University of Mississippi, University, MS, USA
3   H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan
5   Department of Basic Sciences, DHA Suffa University, Karachi, Pakistan
,
Yan-Hong Wang
1   National Center for Natural Products Research, University of Mississippi, University, MS, USA
,
Zulfiqar Ali
1   National Center for Natural Products Research, University of Mississippi, University, MS, USA
,
Troy J. Smillie
1   National Center for Natural Products Research, University of Mississippi, University, MS, USA
,
Ikhlas A. Khan
1   National Center for Natural Products Research, University of Mississippi, University, MS, USA
2   Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, University, MS, USA
4   Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia
› Author Affiliations
Further Information

Publication History

received 27 January 2015
revised 21 September 2015

accepted 08 October 2015

Publication Date:
15 December 2015 (online)

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Abstract

A reversed-phase high-performance liquid chromatography method was developed for the chemical fingerprinting of Commiphora wightii gum resin (guggul). This method was also used for the quantification of E- and Z-guggulsterones in different C. wightii gum resin samples. The analysis was conducted on a high-performance liquid chromatography system with a reversed-phase column using a gradient elution comprised of water and acetonitrile with 0.1 % acetic acid, and a run time of 50 min at 38 °C. The calibration curve of E- and Z-guggulsterones showed good linearity with a standard correlation coefficient > 0.999, which is within the established range (0.5–250 µg/mL). Twelve marker compounds were selected and successfully analyzed by this method in 22 C. wightii gum resin samples along with 9 gum resin samples of possible adulterant species.

Key words

Commiphora wightii - Burseraceae - guggul - HPLC - quantitative analysis
 

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