Planta Med 2016; 82(01/02): 131-137
DOI: 10.1055/s-0035-1557875
Natural Product Chemistry
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Antimicrobial and Antileishmanial Activities of Diterpenoids Isolated from the Roots of Salvia deserta

Jennifer Búfalo
Institute of Biosciences (IB), UNESP – Univ. Estadual Paulista, Botucatu, São Paulo, Brazil
United States Department of Agriculture, Agricultural Research Service, Natural Products Utilization Research Unit, University, Mississippi, U. S. A.
,
Charles L. Cantrell
United States Department of Agriculture, Agricultural Research Service, Natural Products Utilization Research Unit, University, Mississippi, U. S. A.
,
Melissa R. Jacob
National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, The University of Mississippi, University, Mississippi, U. S. A.
,
Kevin K. Schrader
United States Department of Agriculture, Agricultural Research Service, Natural Products Utilization Research Unit, University, Mississippi, U. S. A.
,
Babu L. Tekwani
National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, The University of Mississippi, University, Mississippi, U. S. A.
Department of BioMolecular Sciences, School of Pharmacy, University of Mississippi, University, Mississippi, U. S. A.
,
Tatyana S. Kustova
Institute of Plant Biology and Biotechnology, Timiriazeva, Republic of Kazakhstan
,
Abbas Ali
National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, The University of Mississippi, University, Mississippi, U. S. A.
,
Carmen S. F. Boaro
Institute of Biosciences (IB), UNESP – Univ. Estadual Paulista, Botucatu, São Paulo, Brazil
› Author Affiliations
Further Information

Publication History

received 09 February 2015
revised 16 July 2015

accepted 19 July 2015

Publication Date:
26 August 2015 (eFirst)

Abstract

Four diterpenes with biological activity were isolated from Salvia deserta roots. Taxodione was considered leishmanicidal with an IC50 value of 1.46 µM (0.46 mg/L) against Leishmania donovani and also exhibited antifungal and antimicrobial activities. Ferruginol displayed the greatest activity [24-h IC50 of 4.5 µM (1.29 mg/L)] against the fish pathogenic bacteria Streptococcus iniae. The crude extract fraction that contained the isolated compounds 7-O-acetylhorminone and horminone showed stronger in vitro antibacterial activity (1.3 mg/L for Staphylococcus aureus and 1.1 mg/L for methicillin-resistant S. aureus) than the compounds tested alone. 7-O-Acetylhorminone and horminone exhibited a synergistic effect against methicillin-resistant S. aureus (FIC of 0.2), and horminone had better activity against S. aureus with respect to other compounds isolated from S. deserta roots. In larvicidal bioassays, these extracts and isolated pure compounds did not show any activity at the highest dose of 125 mg/L against 1-d-old Aedes aegypti larvae.

Supporting Information