Planta Medica Letters 2015; 2(01): e39-e41
DOI: 10.1055/s-0035-1557833
Letter
Georg Thieme Verlag KG Stuttgart · New York

Assessment of the Effect of Arbutin Isomers and Kojic Acid on Melanin Production, Tyrosinase Activity, and Tyrosinase Expression in B16-4A5 and HMV-II Melanoma Cells

Hisahiro Kai
Department of Pharmaceutical Health Sciences, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, Miyazaki, Japan
,
Koji Matsuno
Department of Pharmaceutical Health Sciences, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, Miyazaki, Japan
› Author Affiliations
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Publication History

received 14 March 2015
revised 04 June 2015

accepted 18 July 2015

Publication Date:
09 September 2015 (online)

Abstract

The inhibitory effects of α-arbutin, β-arbutin, and kojic acid on melanogenesis, tyrosinase activity, and tyrosinase protein expression in mouse melanoma cells (B16-4A5) and human melanoma cells (HMV-II) were directly compared. β-Arbutin showed a stronger inhibitory effect on melanogenesis and tyrosinase expression in B16-4A5 cells than α-arbutin and kojic acid. Kojic acid showed a stronger inhibitory effect on mushroom and B16-4A5 tyrosinase activity than α-arbutin and β-arbutin. In contrast, kojic acid inhibited all of these effects more strongly than α-arbutin or β-arbutin in HMV-II cells. These results suggest that kojic acid may be used as a positive control for the inhibitory melanogenesis assay, and for tyrosinase activity and tyrosinase expression assays that use HMV-II cells. Moreover, using HMV-II cells with kojic acid as the positive control may facilitate the search for new skin-whitening agents using natural products and provide an alternative to the B16-4A5 assay.

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